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| Product Name | Plant Direct PCR Kit |
| Catalog No. | MDKPK-0024 |
| Description | A plant direct-PCR kit featuring an inhibitor-tolerant DNA polymerase and a simple lysis buffer. Fresh or frozen plant tissues can be lysed and used directly as PCR templates. |
| Intended Use | Plant direct PCR and high-throughput plant screening. |
| Principle / Technology | Engineered DNA polymerase with enhanced tolerance to plant-derived PCR inhibitors, combined with direct tissue lysis. |
| Detection Method | Direct endpoint PCR followed by gel electrophoresis. |
| Sample Type | Fresh or frozen plant leaves and seeds, including tobacco, Arabidopsis, wheat, rice and maize samples. |
| Performance Range / Specifications | Supports DNA targets up to 5 kb. Demonstrated with 0.3–2.3 kb targets from crude plant preparations. |
| Specificity | Maintains strong amplification performance in the presence of plant-derived PCR inhibitors. |
| Components / Formulation | 2× Plant Direct Master Mix and Plant Direct Lysis Buffer. |
| Storage Conditions | Store the 2× master mix at −20°C. Store the lysis buffer at −20°C or 2–8°C. |
| Package Specifications | 200 reactions. |
| Product Form | Plant direct PCR kit. |
| Key Features | No conventional DNA extraction; broad leaf and seed compatibility; inhibitor-tolerant polymerase; stable after 50 freeze-thaw cycles; high-throughput format. |
| Compatibility | Optimized for non-polysaccharide- and non-polyphenol-rich plant samples. |
| Application Notes / Precautions | PCR products are blunt-ended and are compatible with seamless recombination or TOPO-style cloning workflows. |
For research use only, not for clinical use.
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