- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | PIPES Buffer, 0.5 M, pH 6.8, Sterile-Filtered |
| Catalog No. | ATR-B-YJL-0191 |
| Description | A sterile-filtered 0.5 M PIPES buffer solution at pH 6.8, optimized for calcium phosphate-mediated transfection and other cell biology applications requiring precise pH control in the physiological range. PIPES is a zwitterionic ethanesulfonic acid buffer with a pKa of 6.8, making it nearly ideal for maintaining pH near neutrality. The very low metal ion binding affinity of PIPES makes it particularly suitable for experiments involving divalent cations. |
| Intended Use | pH buffering agent for cell culture, calcium phosphate transfection, and biochemical assays requiring metal-ion-independent buffering. |
| Principle / Technology | Zwitterionic pH buffering with negligible metal chelation capacity |
| Detection Method | pH meter verification |
| Sample Type | Cell culture medium preparation, calcium phosphate transfection, enzyme assays sensitive to divalent cations |
| Performance Range / Specifications | Effective buffering range: pH 6.1–7.5; pKa at 20 °C: 6.8 |
| Sensitivity / LOD | pH maintenance within ±0.05 pH units over 24 hr in open culture conditions |
| Specificity | Negligible binding to Ca²⁺, Mg²⁺, Mn²⁺ ensures accurate free metal ion concentrations in buffered solutions |
| Reaction Conditions / Protocol | Use as 10–30 mM final concentration; aseptic technique for cell culture applications |
| Components / Formulation | PIPES buffer (0.5 M, pH 6.8 ± 0.1), sterile-filtered through 0.22 µm membrane |
| Storage Conditions | 2–8 °C |
| Shelf Life | 12 months at 2–8 °C |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid, sterile-filtered in PETG bottle |
| Quality Control | Sterility per USP <71>; endotoxin ≤0.1 EU/mL; pH verified by calibrated meter |
| Key Features | Near-zero metal binding affinity maintains accurate free divalent cation concentrations for electrophysiology and enzyme kinetics studies |
| Purity | ≥99%; DNase/RNase free; sterile-filtered 0.22 µm |
| Concentration | As specified on product label |
| Activity / Unit Definition | pH buffering capacity within ±0.05 pH units of stated value |
| Molecular Weight | Not applicable — inorganic/organic buffer salts |
| Source / Origin | Synthetic, prepared from analytical grade reagents |
| pH Range / Optimal pH | As stated in product specification |
| Shipping Conditions | Ambient temperature; protect from extreme heat |
| Expiration Date / Stability | 12–24 months at recommended storage temperature |
| Regulatory / Compliance | For research use and IVD manufacturing; ISO 13485 quality system |
| Compatibility | Compatible with standard biochemical and cell biology applications |
| Recommended Buffer System | Use as directed; compatible with biological assay systems |
| Application Notes / Precautions | Avoid contamination; use aseptic technique for sterile products; verify pH before critical applications |
| Batch-to-Batch Consistency | pH within ±0.05 units; conductivity within ±5% of reference lot |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
