Phosphoenolpyruvate Carboxylase (PEPC) Activity Assay Kit, 100T/96S
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Phosphoenolpyruvate Carboxylase (PEPC) Activity Assay Kit, 100T/96S

Cat.No: SCAK-YJL-0054 Datasheet

Specification Quantities

100T/96S:
- +
Product Details Background Product Features Product Advantages Related Products
Product Name Phosphoenolpyruvate Carboxylase (PEPC) Activity Assay Kit, 100T/96S
Catalog No. SCAK-YJL-0054
Detection Method Micro-volume method, Spectrophotometer/Microplate Reader
Storage Store at -20°C, 6 months
Intended Use For research use only.
Note For your safety and health, please wear lab coat, disposable gloves and mask during operation.

Phosphoenolpyruvate Carboxylase (PEPC) is a key enzyme widely distributed in plants, algae, bacteria, and some protozoa. It plays a pivotal role in multiple metabolic pathways, with its core function centered on catalyzing the irreversible carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA) and inorganic phosphate (Pi).
In plants, PEPC is critical for photosynthetic carbon fixation in C4 and crassulacean acid metabolism (CAM) plants, enabling efficient CO2 concentration and utilization under various environmental conditions. It also participates in non-photosynthetic processes such as nitrogen metabolism, pH regulation, and organic acid synthesis in both plants and microorganisms.
For research fields including plant physiology, microbial metabolism, agricultural biotechnology, and biochemistry, accurate detection of PEPC activity is essential. It provides critical data for studies on plant stress resistance mechanisms, crop yield improvement, microbial metabolic engineering, and enzyme function characterization.
Traditional PEPC activity detection methods often face challenges such as complex operation steps, low sensitivity, and interference from impurities. The development of specialized assay kits has addressed these limitations, offering standardized, reliable, and efficient solutions for researchers worldwide.

Utilizes a micro-volume detection method compatible with both spectrophotometers and microplate readers, ensuring flexibility for different laboratory setups.
Designed with high specificity for PEPC, minimizing cross-reactivity with other enzymes or biological molecules in sample matrices.
Offers a short assay time, allowing researchers to complete multiple samples efficiently without compromising result quality.
The kit includes all necessary reagents and components for immediate use, reducing the need for additional reagent preparation and minimizing experimental variability.
Storage conditions are straightforward (-20°C) with a 6-month shelf life, ensuring long-term usability and stability of reagents.
Suitable for a wide range of sample types, including plant tissues (leaves, roots, stems), microbial cultures, and cell lysates, expanding its application scope across diverse research projects.

Delivers high sensitivity and accuracy, enabling the detection of low PEPC activity levels in samples with minimal sample input requirements.
Provides consistent and reproducible results across experiments and laboratories, adhering to strict quality control standards for reliable data interpretation.
User-friendly protocol with clear, step-by-step instructions, making it accessible to both experienced researchers and those new to enzyme activity assays.
Cost-effective pricing for 100 tests/96 samples, offering excellent value for high-throughput experiments or large-scale research projects.
Manufactured with high-purity reagents to ensure minimal background noise and optimal reaction efficiency, enhancing the precision of activity quantification.
Complies with standard research laboratory practices, ensuring safety and convenience during operation (when used with appropriate protective equipment).

For research use only, not for clinical use.

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