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| Product Name | Phosphate-Buffered Saline with Tween 20 (PBST), 10x Concentrate |
| Catalog No. | ATR-B-YJL-0174 |
| Description | A ten-fold concentrated phosphate-buffered saline with Tween 20 surfactant, providing an alternative wash buffer system for immunoassays requiring phosphate-based ionic strength. |
| Intended Use | Wash buffer for ELISA, bead-based immunoassays, and immunohistochemistry where phosphate buffering is the established protocol standard. |
| Principle / Technology | Phosphate buffers at neutral pH with Tween 20 reducing surface tension and non-specific protein binding. |
| Detection Method | pH electrode, surface tension measurement |
| Sample Type | ELISA microplates, IHC tissue sections, Luminex bead assays |
| Performance Range / Specifications | pH 7.2–7.6 after dilution |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Dilute 1:10 with deionized water; typical wash volume 200-300 µL per well for ELISA. |
| Components / Formulation | Sodium chloride, potassium chloride, sodium phosphate dibasic, potassium phosphate monobasic, Tween 20 in ultrapure water |
| Storage Conditions | 15–30°C |
| Shelf Life | 24 months |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid concentrate |
| Quality Control | pH, osmolality, detergent concentration verification |
| Key Features | Standard for ELISA washing; economical concentrate; consistent detergent content |
For research use only, not for clinical use.
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