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| Product Name | Phosphate Buffer, 0.1 M, pH 7.0 |
| Catalog No. | ATR-B-YJL-0185 |
| Description | A defined molarity phosphate buffer at neutral pH, free of sodium chloride, used for applications requiring precise phosphate concentration without additional ionic components. |
| Intended Use | Enzyme kinetic studies, protein stability assays, and as a mobile phase component in biomolecule chromatography where chloride ions may interfere. |
| Principle / Technology | Equilibrium mixture of monobasic (H2PO4-) and dibasic (HPO4 2-) phosphate ions providing buffering at neutral pH with pKa of 7.20. |
| Detection Method | pH electrode, conductivity measurement, ion chromatography |
| Sample Type | Purified enzymes, protein solutions, chromatography samples |
| Performance Range / Specifications | pH 6.8–7.2, ionic strength 0.15 M |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Use undiluted or dilute as required; adjust to final experimental pH with NaOH or HCl if needed. |
| Components / Formulation | Sodium phosphate monobasic, sodium phosphate dibasic in ultrapure water, no NaCl or preservatives |
| Storage Conditions | 15–30°C |
| Shelf Life | 6 months; filter before use to remove any microbial growth |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid |
| Quality Control | pH verification, phosphate concentration by spectrophotometric molybdate assay |
| Key Features | Salt free (no NaCl); defined ionic strength; suitable for enzyme kinetics; compatible with chromatography |
For research use only, not for clinical use.
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