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| Product Name | One-Step RT-qPCR SYBR Green Kit with gDNA Removal |
| Catalog No. | MDKPK-0037 |
| Description | A dye-based one-step RT-qPCR kit combining high-sensitivity amplification, genomic DNA removal and dUTP/UDG carryover contamination control. |
| Intended Use | Gene quantification, gene-expression analysis and virus detection. |
| Principle / Technology | Warm-start reverse transcription and antibody-regulated Taq polymerase operate in an optimized SYBR Green buffer. A genomic DNA removal mix reduces residual DNA, and dUTP/UDG chemistry limits carryover contamination. |
| Detection Method | SYBR Green dye-based real-time RT-qPCR with melt-curve analysis. |
| Sample Type | RNA templates that may contain residual genomic DNA. |
| Performance Range / Specifications | 250 reactions; compatible with targets of varied GC content; includes a universal passive reference dye for broad instrument use. |
| Specificity | The optimized enzyme system supports single-peak melt curves in specificity testing, while the genomic DNA removal step limits DNA-derived background. |
| Reaction Conditions / Protocol | Treat RNA with the supplied genomic DNA removal mix when needed, then combine with the 2× SYBR Green master mix, primers and template for one-step RT-qPCR. |
| Components / Formulation | 2× one-step SYBR Green master mix containing tracking dye and universal ROX reference dye; 5× genomic DNA removal mix; dUTP/UDG carryover-control chemistry. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 250 reactions. |
| Product Form | One-step dye-based RT-qPCR kit with genomic DNA removal module. |
| Quality Control | Sensitivity, melt-curve specificity, carryover-control efficiency and genomic DNA removal are evaluated. |
| Key Features | High sensitivity and specificity; dUTP/UDG contamination control; genomic DNA removal; universal instrument compatibility. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Designed for real-time PCR instruments used for SYBR Green detection. |
| Application Notes / Precautions | Evaluate melt curves for nonspecific products and primer dimers. Use the genomic DNA removal step for RNA samples with potential DNA carryover. |
For research use only, not for clinical use.
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