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| Product Name | One-Step RT-qPCR Probe Kit with Thermolabile UDG |
| Catalog No. | MDKPK-0033 |
| Description | A probe-based one-step RT-qPCR kit combining sensitive RNA detection with a thermolabile UDG carryover-control system. |
| Intended Use | Virus detection, pathogen testing and gene-expression analysis. |
| Principle / Technology | Thermolabile UDG degrades uracil-containing double-stranded carryover DNA at room temperature and rapidly becomes inactive at 50–55°C, preserving newly generated cDNA during reverse transcription and amplification. |
| Detection Method | Fluorescent probe-based real-time RT-qPCR. |
| Sample Type | RNA templates, including viral and cellular RNA. |
| Performance Range / Specifications | 250 reactions at 20 µL per reaction; supports multiplex probe detection. |
| Sensitivity / LOD | Reported detection reached single-digit RNA copy levels or 0.1 pg total RNA. |
| Specificity | Optimized reverse-transcription and hot-start PCR chemistry supports specific amplification without loss of efficiency from the carryover-control system. |
| Reaction Conditions / Protocol | Prepare a 20 µL one-step reaction using assay-specific primers/probes, RNA template and the supplied reaction components. |
| Components / Formulation | One-step probe RT-qPCR master mix containing dUTP and thermolabile UDG, plus reverse-transcription and hot-start PCR enzyme components. |
| Package Specifications | 250 reactions (20 µL per reaction). |
| Product Form | Two-component one-step RT-qPCR probe kit. |
| Quality Control | Low-copy sensitivity, multiplex amplification and UDG carryover-control performance are evaluated. |
| Key Features | Thermolabile UDG carryover control; multiplex probe support; high sensitivity; one-tube reverse transcription and qPCR. |
| Compatibility | Suitable for probe-based real-time PCR instruments. |
| Application Notes / Precautions | Use uracil-containing amplification chemistry for effective carryover control and avoid opening completed qPCR tubes in the reaction setup area. |
For research use only, not for clinical use.
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