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| Product Name | One-Step Mouse Genotyping PCR Kit V2 |
| Catalog No. | MDKPK-0022 |
| Description | A rapid direct-PCR kit for mouse genotyping that combines a short tissue lysis workflow with a hot-start Taq master mix. Crude lysates can be used directly as PCR templates without DNA purification. |
| Intended Use | Mouse genotype identification, mouse genotyping and direct PCR. |
| Principle / Technology | Rapid thermal lysis releases genomic DNA, followed by hot-start Taq amplification directly from the clarified lysate. |
| Detection Method | Direct endpoint PCR followed by gel electrophoresis. |
| Sample Type | Mouse tail, ear, toe, liver, brain, spleen and kidney tissues; blood; 293 cells; plant leaves. |
| Performance Range / Specifications | Three-minute lysis; extension at 15 sec/kb; supports targets up to 8 kb and 4–6-plex amplification. An 8.6 kb target was demonstrated. |
| Specificity | Antibody-mediated hot-start polymerase supports specific amplification from crude lysates. |
| Reaction Conditions / Protocol | Lyse mouse samples at 95°C for 3 minutes, clarify the lysate, then add primers and lysate to the 2× master mix for PCR. |
| Components / Formulation | Mouse Direct Lysis Buffer A, Mouse Direct Lysis Buffer B and 2× rapid hot-start Taq master mix with blue loading dye. |
| Storage Conditions | Store the kit at −30 to −15°C. For long-term storage, keep Mouse Direct Lysis Buffer A and B at 2–8°C. |
| Package Specifications | 200 reactions. |
| Product Form | Direct PCR genotyping kit. |
| Key Features | Three-minute lysis; no DNA purification; broad tissue and sample compatibility; up to 8 kb targets; 4–6-plex PCR; direct gel loading. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | Compatible with crude lysates from multiple mouse tissues, whole blood, cultured cells and plant leaves. |
| Application Notes / Precautions | If crude lysate contains excessive inhibitors, dilute the lysate 2- to 10-fold before PCR. |
For research use only, not for clinical use.
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