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| Product Name | One-Step Mouse Genotyping PCR Kit |
| Catalog No. | MDKPK-0023 |
| Description | A complete mouse genotyping workflow combining proteinase K-based tissue lysis and a blue-dye PCR master mix. Tissue lysates are used directly for amplification without conventional DNA purification. |
| Intended Use | Rapid mouse genotyping and direct PCR from mouse tissue lysates. |
| Principle / Technology | Proteinase K-based crude tissue lysis followed by direct high-yield Taq PCR amplification. |
| Detection Method | Direct endpoint PCR followed by gel electrophoresis. |
| Sample Type | Mouse tail, ear and toe tissues; rat and mouse samples are supported. |
| Performance Range / Specifications | Suitable for targets up to 2 kb and multiplex PCR with up to four primer pairs. Demonstrated with 17 genes ranging from 200 to 800 bp. |
| Specificity | Designed for specific, high-yield amplification directly from crude tissue lysates. |
| Components / Formulation | Proteinase K, mouse tissue lysis buffer, 2× high-yield Taq master mix with blue loading dye and MgCl2 solution. |
| Storage Conditions | Store mouse tissue lysis buffer at 4°C and all other components at −20°C. Store Proteinase K at −20°C. |
| Package Specifications | 200 reactions. |
| Product Form | Direct PCR genotyping kit. |
| Key Features | No DNA purification; reduced handling and contamination risk; direct gel loading; multiplex PCR support; stable master mix. |
| Compatibility | Compatible with mouse and rat genotyping workflows. |
| Application Notes / Precautions | The supplied 2× master mix contains 1.5 mM Mg2+. If amplification is weak, MgCl2 may be increased in 0.5 mM increments. |
For research use only, not for clinical use.
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