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| Product Name | Nuclear and Cytoplasmic Protein Extraction Kit |
| Catalog No. | REKI-0003 |
| Description | Nuclear and cytoplasmic proteins carry distinct information for cell research and proteomics, so workflows often require them separated rather than pooled. This kit first swells and lyses cells with a hypotonic cytoplasmic extraction reagent, yielding the cytoplasmic fraction after a single centrifugation, then extracts nuclear proteins with dedicated reagents A and B. The resulting fractions are ready for SDS-PAGE, Western blotting and EMSA, giving clean compartment-specific readouts. |
| Intended Use | Separation of nuclear and cytoplasmic protein fractions from cells or tissues for SDS-PAGE, Western blotting and EMSA |
| Principle / Technology | Hypotonic swelling releases the cytoplasmic fraction, and dedicated nuclear extraction reagents recover nuclear proteins |
| Detection Method | Cytoplasmic and nuclear protein fractions |
| Sample Type | Cultured animal cells and animal tissues |
| Performance Range / Specifications | Clean fractionation with low cross-contamination between compartments; 50 or 100 preparations per kit |
| Specificity | Compartment-specific recovery of nuclear and cytoplasmic proteins |
| Reaction Conditions / Protocol | Lyse cells in the hypotonic cytoplasmic reagent, centrifuge to collect the cytoplasmic fraction, then extract the nuclear pellet with reagents A and B |
| Components / Formulation | Cytoplasmic extraction reagent, nuclear extraction reagents A and B; sized for 50 or 100 preparations |
| Storage Conditions | -25 to -15°C for 12 months |
| Shelf Life | 12 months |
| Package Specifications | 50 tests; 100 tests |
| Product Form | Ready-to-use reagent set |
| Quality Control | Fractionation purity confirmed by compartment marker analysis on each lot |
| Key Features | Two-step fractionation with simple centrifugation; ready-to-use reagents; direct compatibility with SDS-PAGE, Western blotting and EMSA |
| Source / Origin | Formulated kit |
| Shipping Conditions | Wet ice |
| Expiration Date / Stability | 12 months at -25 to -15°C |
| Regulatory / Compliance | For research use only |
| Compatibility | Fractions feed directly into SDS-PAGE, Western blotting and EMSA |
| Recommended Buffer System | Hypotonic cytoplasmic extraction reagent plus dedicated nuclear extraction reagents A and B |
| Application Notes / Precautions | Keep reagents chilled during use; follow the protocol timing closely to limit cross-contamination between fractions |
| Batch-to-Batch Consistency | Lot-numbered packaging; lot record retained for traceability |
| Starting Material / Tissue Site | Animal cells or tissues supplied by the user |
| Delivery Standard | Standard catalogue shipment, packaging integrity verified before dispatch |
| Readouts / Downstream Assays | SDS-PAGE, Western blotting, EMSA, compartment-specific protein localisation studies |
For research use only, not for clinical use.
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