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| Product Name | Neutral Invertase (NI) Activity Assay Kit, 50T/24S |
| Catalog No. | SCAK-YJL-0019 |
| Detection Method | Visible spectrophotometry, Spectrophotometer |
| Storage | Store at 2-8°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Neutral invertase (NI) is a sucrose-cleaving enzyme in plants with a pH optimum near neutrality (pH ~7.0). It hydrolyzes sucrose into glucose and fructose, playing distinct roles from vacuolar acid invertase in cytosolic sugar metabolism, osmoregulation, and sugar signaling. NI activity is involved in growth, development, and responses to environmental stimuli. This kit measures NI activity at a controlled neutral pH by quantifying the reducing sugars (glucose and fructose) produced from sucrose using a sensitive colorimetric method. |
| Product Application | • Research on sucrose metabolism and hexose-mediated signaling in plant growth and development. • Studies of plant responses to abiotic stresses like drought, salinity, and cold, where osmotic adjustment is key. • Analysis of sink tissue metabolism and phloem unloading mechanisms. • Investigation of sugar metabolism in non-photosynthetic plant tissues. |
| Product Advantages | • pH-Specific Assay: The buffer system is optimized to selectively measure NI activity at pH 7.0, effectively excluding interference from vacuolar acid invertase. • Sensitive Detection: Measures total hexose production, offering high sensitivity to detect activity in various tissues. • Plant-Tailored: Includes specific recommendations for preparing active enzyme extracts from different plant organs. • Straightforward Workflow: A clear, fixed-endpoint assay format simplifies operation and data analysis. |
For research use only, not for clinical use.
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