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| Product Name | Multiplex PCR Amplification Kit |
| Catalog No. | MDKPK-0029 |
| Description | A complete multiplex PCR kit designed for stable, uniform and specific amplification of multiple DNA targets within a single reaction. |
| Intended Use | Multiplex PCR; multi-locus testing of limited samples; amplification of high-GC targets. |
| Principle / Technology | A Taq-based multiplex PCR system combines an optimized reaction buffer with a GC-enhancing additive to support balanced amplification of multiple primer pairs. |
| Detection Method | Endpoint PCR followed by agarose-gel electrophoresis or other assay-specific detection. |
| Sample Type | Genomic DNA and other compatible DNA templates, including low-input samples. |
| Performance Range / Specifications | 200 or 1,000 reactions; demonstrated 19-plex or higher amplification; tested with approximately 1–500 ng genomic DNA and amplicons from about 70 to 916 bp in one multiplex panel. |
| Sensitivity / LOD | Reported multiplex amplification sensitivity reached 1 ng genomic DNA. |
| Specificity | The optimized buffer suppresses nonspecific products and reduces target preference among primer pairs. |
| Reaction Conditions / Protocol | Prepare the reaction using the supplied multiplex PCR reagents. Recommended primer design includes 21–30 bp primers, 40%–60% GC content and annealing temperatures of approximately 68°C or higher; typical final primer concentration is 0.1–0.2 µM per primer. |
| Components / Formulation | Multiplex PCR amplification reagents with a supplied GC enhancer. |
| Storage Conditions | Store all components at −20°C. |
| Shelf Life | One year under the stated storage conditions. |
| Package Specifications | 200 reactions; 1,000 reactions. |
| Product Form | Complete multiplex PCR reagent kit. |
| Quality Control | Multiplex level, target uniformity, sensitivity, specificity and amplicon-length compatibility are evaluated. |
| Key Features | 19-plex or higher capability; stable target balance; broad GC and amplicon-length compatibility; supplied GC enhancer; low-template detection. |
| Compatibility | Suitable for standard multiplex endpoint PCR and products requiring 3′ A overhangs for T-vector cloning. |
| Application Notes / Precautions | Use primer pairs with verified singleplex performance. If targets are missing or imbalanced, adjust primer concentration, cycle number, extension time or gel resolution. |
For research use only, not for clinical use.
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