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| Product Name | Mouse Neutrophil Isolation Kit ( Negative Selection ) |
| Catalog No. | SM-HMM-0047 |
| Description | The principle of magnetic beads cell isolation is to use immunomagnetic beads to label cells, the cells bound to the magnetic beads and without bound to the magnetic beads are separated by an external magnetic field. Our Cell Isolation Kit has two separation technologies, negative selection and positive selection, for both human and mouse samples, and is suitable for different application directions. |
| Features | Easy and fast operation: no need for separation column, used with magnetic separator, operation can be completed in as fast as 30min; High activity and purity: the target cells are free of antibody and magnetic bead labeling, and the purity is up to 95%. |
| Storage | Store at 2-8°C, do not freeze. |
| Applications | This kit is suitable for sorting mouse bone marrow, peripheral blood or spleen neutrophils. |
| Product Introduction | Isolating viable, functional neutrophils is challenging due to their sensitivity to activation and short lifespan. This kit employs a refined negative selection strategy to purify untouched neutrophils from mouse bone marrow, the primary source. By using a biotin-antibody cocktail against non-neutrophil cells (lymphocytes, monocytes, erythroid cells) followed by anti-biotin magnetic bead depletion, it yields a population of neutrophils that have not been activated by antibody binding. This is critical for studies of chemotaxis, phagocytosis, NETosis, and oxidative burst where baseline activation state must be preserved. |
| Product Application | • Functional studies of neutrophil migration (chemotaxis), phagocytosis, and killing. • Research on neutrophil extracellular trap (NET) formation. • Analysis of oxidative burst and degranulation responses. • Adoptive transfer experiments in models of infection or sterile inflammation. |
| Product Advantages | • Preserved Function & Purity: Isolates neutrophils without antibody labeling, minimizing pre-activation and ensuring cells are responsive in functional assays, with typical purities >85-90%. • Optimized for Bone Marrow: The protocol is specifically designed and validated for the complex mixture of cells in mouse bone marrow, where neutrophils are most abundant. • Maintains Cell Viability: The rapid, cold-processing workflow is designed to minimize stress and maintain high cell viability for downstream functional applications. • Ideal for Sensitive Assays: The untouched nature of the cells makes them suitable for measuring subtle changes in activation markers or for transcriptional profiling without isolation-induced artifacts. |
For research use only, not for clinical use.
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