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| Product Name | MOPS Running Buffer, 20× Concentrate |
| Catalog No. | ATR-B-YJL-0189 |
| Description | A concentrated 20× MOPS-based electrophoresis running buffer formulated for denaturing agarose gel electrophoresis of RNA. The buffer system maintains a consistent pH of 7.0 during electrophoresis, providing optimal resolution of RNA species from 0.5 to 10 kb. When diluted to 1×, the buffer delivers uniform migration without the pH drift commonly observed with Tris-acetate or Tris-borate systems during extended electrophoresis runs. |
| Intended Use | Denaturing agarose gel electrophoresis of RNA using MOPS buffer for formaldehyde-based gel systems. |
| Principle / Technology | MOPS zwitterionic buffer maintaining neutral pH during electrophoresis |
| Detection Method | Ethidium bromide or SYBR staining with UV or blue-light imaging |
| Sample Type | Total RNA and mRNA samples on formaldehyde-containing agarose gels |
| Performance Range / Specifications | 1× working solution: 20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA, pH 7.0 |
| Sensitivity / LOD | RNA bands resolved with molecular weight linearity up to 10 kb |
| Specificity | MOPS buffer optimized for RNA secondary structure denaturation in formaldehyde gel systems |
| Reaction Conditions / Protocol | Dilute 1:20 with DEPC-treated water; use as gel preparation and running buffer |
| Components / Formulation | 20× MOPS concentrate (400 mM MOPS, 100 mM sodium acetate, 20 mM EDTA, pH adjusted to 7.0 with NaOH) |
| Storage Conditions | Room temperature; protect from light; yellowing indicates degradation |
| Shelf Life | 18 months at room temperature in dark storage |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid concentrate, clear to faint yellow |
| Quality Control | Each lot verified for pH and conductivity; absence of RNase activity confirmed by incubation with RNA substrate |
| Key Features | Twenty-fold concentration reduces storage footprint and shipping cost compared to ready-to-use buffer |
| Purity | ≥99%; DNase/RNase free; sterile-filtered 0.22 µm |
| Concentration | As specified on product label |
| Activity / Unit Definition | pH buffering capacity within ±0.05 pH units of stated value |
| Molecular Weight | Not applicable — inorganic/organic buffer salts |
| Source / Origin | Synthetic, prepared from analytical grade reagents |
| pH Range / Optimal pH | As stated in product specification |
| Shipping Conditions | Ambient temperature; protect from extreme heat |
| Expiration Date / Stability | 12–24 months at recommended storage temperature |
| Regulatory / Compliance | For research use and IVD manufacturing; ISO 13485 quality system |
| Compatibility | Compatible with standard biochemical and cell biology applications |
| Recommended Buffer System | Use as directed; compatible with biological assay systems |
| Application Notes / Precautions | Avoid contamination; use aseptic technique for sterile products; verify pH before critical applications |
| Batch-to-Batch Consistency | pH within ±0.05 units; conductivity within ±5% of reference lot |
For research use only, not for clinical use.
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