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Micro β-amylase Assay Kit, 100T/48S

Cat.No: SCAK-YJL-0212 Datasheet

Specification Quantities

100T/48S:
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Product Details Product Overview Related Products
Product Name Micro β-amylase Assay Kit, 100T/48S
Catalog No. SCAK-YJL-0212
Detection Method Micro-volume method, Spectrophotometer/Microplate Reader
Storage Store at 2-8°C, 6 months
Intended Use For research use only.
Note For your safety and health, please wear lab coat, disposable gloves and mask during operation.
Product Introduction This micro-amylase assay kit is specifically designed for the sensitive quantification of α-amylase activity in small sample volumes, such as micro-dialysates, single-cell lysates, or precious clinical samples. It utilizes a chromogenic substrate (such as a dyed starch derivative or a blocked p-nitrophenyl maltoheptaoside) that, upon hydrolysis by amylase, releases a colored product measurable at a specific wavelength. The optimized chemistry allows for high sensitivity while minimizing sample consumption.
Product Application • Measurement of amylase in saliva, pancreatic juice, or tears where sample volume is limited.
• Research on localized enzyme secretion or activity in tissue micro-environments.
• High-throughput screening of enzyme inhibitors using small reaction volumes in 384-well plates.
• Monitoring amylase activity in fermentation broths or enzyme purification fractions.
Product Advantages • Ultra-Low Sample Requirement: Requires only 1-10 µL of sample per test, conserving valuable biological material.
• Enhanced Sensitivity: The use of optimized chromogenic substrates provides a lower limit of detection compared to traditional iodine-starch methods.
• Wide Dynamic Range: Capable of accurately measuring activity across a broad concentration range, from very low to high amylase levels.
• Adaptable Format: Suitable for both micro-cuvette and microplate readers, offering flexibility for different laboratory setups and throughput needs.

For research use only, not for clinical use.

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