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| Product Name | MES Buffer, 1 M, pH 6.0, Sterile-Filtered |
| Catalog No. | ATR-B-YJL-0194 |
| Description | 1 M MES (2-(N-morpholino)ethanesulfonic acid) buffer solution, pH-adjusted to 6.0 with NaOH and sterile-filtered through 0.22 µm membrane. MES is a zwitterionic Good's buffer with pKa 6.15 at 25 °C, providing optimal buffering capacity in the pH 5.5-6.7 range. Widely used in protein crystallization, enzymatic assays requiring slightly acidic conditions, and plant cell culture media. |
| Intended Use | Buffering agent for enzymatic reactions at pH 5.5-6.7; protein crystallization screening; plant tissue culture media; yeast and fungal culture; chromatography running buffer; fluorescent protein (GFP) pH-stability assays. |
| Principle / Technology | MES is a zwitterionic N-substituted aminosulfonic acid; minimal metal ion complexation (unlike phosphate or citrate); does not interfere with most enzymatic reactions; pKa 6.15 at 25 °C, ΔpKa/°C = -0.011 |
| Detection Method | Dilute to desired working concentration (typically 10-100 mM); adjust pH if necessary after dilution; compatible with most biological systems at working concentrations |
| Components / Formulation | MES free acid (CAS 4432-31-9), NaOH for pH adjustment to 6.0, ultrapure water; 0.22 µm sterile-filtered |
| Storage Conditions | 15-25 °C; keep container tightly closed; protect from light (MES solutions may yellow with prolonged light exposure) |
| Shelf Life | 24 months from manufacture date in unopened container; use within 12 months after opening |
| Package Specifications | 100 mL, 500 mL, 1 L sterile HDPE bottles |
| Product Form | Clear, colorless sterile liquid, ready-to-use after dilution |
| Key Features | Sterile-filtered 0.22 µm; 1 M concentrate for convenient dilution; minimal metal chelation; does not absorb UV at >240 nm — suitable for spectrophotometric enzyme assays; non-toxic to most cell types; pH stable for months at RT |
| Purity | MES purity ≥99.5% by titration; heavy metals <5 ppm; DNase/RNase tested free; endotoxin <0.05 EU/mL |
| Concentration | 1.00 ± 0.02 M MES, pH 6.0 ± 0.05 at 25 °C |
| Activity / Unit Definition | pH buffering capacity within ±0.05 pH units of stated value |
| Molecular Weight | Not applicable — inorganic/organic buffer salts |
| Source / Origin | Synthetic, prepared from analytical grade reagents |
| pH Range / Optimal pH | pH 6.0 ± 0.05 at 25 °C; effective pH range 5.5-6.7; ΔpH/°C = -0.011 |
| Shipping Conditions | Ambient temperature; sterile packaging — if seal is broken, filter before use |
| Expiration Date / Stability | 24 months unopened; 12 months after opening; discard if solution yellows significantly or microbial growth observed; verify pH before use if stored >6 months |
| Regulatory / Compliance | For laboratory and research use only; RUO; not classified as dangerous goods; ISO 9001 |
| Compatibility | Compatible with most enzymatic reactions; minimal inhibition of kinases, phosphatases, and restriction enzymes; not suitable for bicarbonate/CO₂-buffered cell culture systems; compatible with metal ions (Mg²⁺, Ca²⁺, Mn²⁺) — MES does not complex divalent cations significantly |
| Recommended Buffer System | Use as directed; compatible with biological assay systems |
| Application Notes / Precautions | Avoid contamination; use aseptic technique for sterile products; verify pH before critical applications |
| Batch-to-Batch Consistency | pH within ±0.05 units; conductivity within ±5% of reference lot |
For research use only, not for clinical use.
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