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| Product Name | Malic Acid Synthase (MS) Activity Assay Kit, 50T/24S |
| Catalog No. | SCAK-YJL-0039 |
| Detection Method | Visible spectrophotometry |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Malic acid synthase (MS) is a key enzyme in the glyoxylate cycle, catalyzing the condensation of acetyl-CoA and glyoxylate to form malate. This pathway bypasses the decarboxylation steps of the TCA cycle, enabling net conversion of fatty acids or acetate into carbohydrates, a critical process in germinating oilseeds, certain microorganisms, and tumor metabolism. This kit quantitatively measures MS activity by coupling the CoA release to a colorimetric detection system, providing a sensitive and direct method for enzyme analysis in plant and microbial extracts. |
| Product Application | • Study of the glyoxylate cycle and gluconeogenesis in germinating seeds, seedlings, and plant tissues. • Research on microbial metabolism, including roles in pathogenesis, persistence, and biofuel production. • Investigation of metabolic reprogramming in cancer cells and other contexts where acetate utilization is important. • Biochemical characterization of MS enzymes from recombinant or native sources. |
| Product Advantages | • Specific Coupled Assay: The reaction directly measures CoA production, ensuring specificity for MS activity over other enzymes. • Sensitive Detection: The colorimetric amplification allows for precise measurement even in samples with low enzyme levels or activity. • Optimized for Complex Samples: Formulated to work effectively with crude plant homogenates and microbial lysates that may contain interfering substances. • Straightforward Procedure: A clear, step-by-step protocol minimizes setup time and technical variability. |
For research use only, not for clinical use.
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