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| Product Name | Long-Range One-Step RT-PCR Kit with Loading Dye |
| Catalog No. | MDKPK-0043 |
| Description | A one-step endpoint RT-PCR kit containing loading dye for direct electrophoresis after amplification. |
| Intended Use | Virus detection, gene-expression analysis and endpoint PCR testing of RNA templates. |
| Principle / Technology | Reverse transcription and PCR occur sequentially in one tube. The reaction mix contains buffer, dNTPs and loading dye for direct gel application. |
| Detection Method | Endpoint RT-PCR followed by direct agarose-gel electrophoresis. |
| Sample Type | Total RNA, viral RNA and other compatible RNA templates. |
| Performance Range / Specifications | 50 reactions at 50 µL per reaction; supports amplicons longer than 10 kb; completed products can be loaded directly onto an agarose gel. |
| Sensitivity / LOD | Reported detection reaches approximately 1 pg total RNA. |
| Specificity | One-tube processing reduces handling and contamination risk. |
| Reaction Conditions / Protocol | Combine RNA, gene-specific primers, one-step dye-containing mix and enzyme components in a 50 µL reaction, then load the completed product directly for gel analysis. |
| Components / Formulation | 2× one-step reaction mix containing optimized buffer, dNTPs and loading dye, supplied with the corresponding reverse-transcription/PCR enzyme blend. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 50 reactions (50 µL per reaction). |
| Product Form | Two-component one-step RT-PCR kit with loading dye. |
| Quality Control | Sensitivity, long-fragment performance and direct gel-loading compatibility are evaluated. |
| Key Features | One-tube RT-PCR; long-fragment amplification; direct gel loading; reduced handling and contamination risk. |
| Compatibility | Suitable for conventional thermal cyclers and agarose-gel electrophoresis. |
| Application Notes / Precautions | Use gene-specific primers and optimize extension time for long targets. |
For research use only, not for clinical use.
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