- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Long-Range One-Step RT-PCR Kit |
| Catalog No. | MDKPK-0042 |
| Description | A one-step endpoint RT-PCR kit that performs reverse transcription and PCR in the same tube and supports long-fragment amplification. |
| Intended Use | Virus detection, gene-expression analysis and endpoint PCR testing of RNA templates. |
| Principle / Technology | Gene-specific reverse transcription and PCR amplification are completed sequentially in one closed tube using an optimized master mix and enzyme blend. |
| Detection Method | Endpoint RT-PCR followed by agarose-gel electrophoresis. |
| Sample Type | Total RNA, viral RNA and other compatible RNA templates. |
| Performance Range / Specifications | 50 reactions at 50 µL per reaction; supports amplicons longer than 10 kb, with a reported successful product of 12.1 kb. |
| Sensitivity / LOD | Product summary reports sensitivity to 1 pg total RNA; a reported cellular RNA experiment detected 0.1 pg for a 555 bp target. |
| Specificity | Closed-tube processing and optimized enzyme chemistry reduce handling and contamination risk. |
| Reaction Conditions / Protocol | Combine RNA, gene-specific primers, 2× one-step mix and enzyme mix in a 50 µL reaction and run a suitable reverse-transcription/PCR program. |
| Components / Formulation | 2× one-step mix containing optimized buffer and dNTPs; enzyme mix containing reverse transcriptase, RNase inhibitor and DNA polymerase. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 50 reactions (50 µL per reaction). |
| Product Form | Two-component one-step RT-PCR kit. |
| Quality Control | Low-input sensitivity and long-fragment amplification are evaluated. |
| Key Features | One-tube workflow; reduced contamination risk; sensitive RNA detection; amplicons longer than 10 kb. |
| Compatibility | Suitable for conventional thermal cyclers and endpoint gel analysis. |
| Application Notes / Precautions | Use gene-specific primers. High-quality intact RNA and adequate extension time are important for long targets. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
