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| Product Name | Long-Range DNA Polymerase with Mg2+ Buffer |
| Catalog No. | MDKPK-0020 |
| Description | A long-range DNA polymerase system combining Taq activity and a proofreading component, supplied with magnesium-containing buffer and a PCR enhancer for difficult high-GC targets. |
| Intended Use | Long-range PCR, amplification of variable-length templates and high-GC PCR. |
| Principle / Technology | Taq DNA polymerase blended with a 3′→5′ exonuclease-containing protein and optimized for long-range amplification. |
| Detection Method | Endpoint PCR followed by gel electrophoresis. |
| Sample Type | Human, rice, soybean and cotton genomic DNA; bacterial colonies; high-GC templates. |
| Performance Range / Specifications | Demonstrated amplification of 3.6–21.0 kb genomic targets with GC contents from 27% to 78%. |
| Sensitivity / LOD | Demonstrated amplification of 11.5 kb and 17.2 kb targets from 0.5–500 ng cotton genomic DNA. |
| Specificity | Reported fidelity is approximately sixfold higher than standard Taq DNA polymerase. |
| Components / Formulation | Long-range DNA polymerase, Mg2+-containing PCR buffer and PCR enhancer. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 500 U. |
| Product Form | Long-range DNA polymerase system. |
| Key Features | Up to 21 kb genomic amplification; broad GC tolerance; strong sensitivity; enhancer supplied for high-GC targets. |
| Compatibility | PCR products carry 3′-A overhangs and are suitable for T-vector cloning. |
| Application Notes / Precautions | Use the supplied enhancer when high-GC or structurally complex templates are difficult to amplify. |
For research use only, not for clinical use.
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