- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | α-Ketoglutarate Dehydrogenase (α-KGDH) Activity Assay Kit, 50T/48S |
| Catalog No. | SCAK-YJL-0176 |
| Detection Method | UV spectrophotometry, Spectrophotometer |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Alpha-ketoglutarate dehydrogenase (α-KGDH) is a key regulatory enzyme complex (Complex II of the TCA cycle) that catalyzes the irreversible conversion of α-ketoglutarate to succinyl-CoA. This reaction is a major site of NADH and CO₂ production and is highly sensitive to oxidative damage and cellular redox status. Dysfunction of α-KGDH is implicated in neurodegenerative diseases, ischemia-reperfusion injury, and other conditions. This kit measures α-KGDH activity by directly monitoring the reduction of NAD⁺ to NADH at 340 nm, providing a clean and continuous kinetic readout. |
| Product Application | • Assessment of mitochondrial respiratory chain function and TCA cycle flux. • Research on neurodegenerative diseases (e.g., Alzheimer's, Parkinson's), where α-KGDH is often impaired. • Investigation of oxidative stress, as the enzyme complex is a known target of reactive oxygen species (ROS). • Studies of metabolic reprogramming in cancer and other proliferative cells. |
| Product Advantages | • Direct UV Kinetic Assay: Measures the primary product (NADH) in real-time, offering high specificity and accurate kinetic parameters (Vmax, Km). • High Sensitivity: UV detection allows measurement of activity in small amounts of tissue, such as brain biopsies or cultured cells. • Mitochondrial Focus: The assay conditions are optimized for activity in mitochondrial extracts, reflecting physiological function. • Reproducible Performance: The straightforward protocol yields highly consistent results run-to-run. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagenoTech. All rights reserved.