Ionic Bound Pectin (ISP) Content Assay Kit, 100T/48S

Cat.No: SCAK-YJL-0059 Datasheet

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100T/48S:

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Product Details Product Overview Related Products

Product Name	Ionic Bound Pectin (ISP) Content Assay Kit, 100T/48S
Catalog No.	SCAK-YJL-0059
Detection Method	Micro-volume method, Spectrophotometer/Microplate Reader
Storage	Store at 2-8°C, 6 months
Intended Use	For research use only.
Note	For your safety and health, please wear lab coat, disposable gloves and mask during operation.

Product Introduction	Pectin is a complex polysaccharide and a major component of the plant cell wall's middle lamella, influencing cell adhesion, tissue integrity, and fruit ripening. Ionic-bound pectin is pectin cross-linked via calcium bridges and other ions; its solubility and content change dramatically during fruit softening and processing. This kit specifically extracts and quantifies the ionic-bound pectin fraction using a chelating agent (e.g., CDTA or EDTA) followed by colorimetric determination of uronic acid content, the main constituent of pectin.
Product Application	• Studies of fruit ripening, softening, and post-harvest quality. • Research on plant cell wall architecture and remodeling. • Food science investigations into gelling properties and texture of fruit-based products. • Biomass characterization for biorefinery applications.
Product Advantages	• Fraction-Specific Analysis: Targets the ionic-bound pectin pool specifically, providing more detailed information than total pectin assays and reflecting changes in cell wall cross-linking. • Specific Chelation Extraction: Uses a standardized chelating agent to selectively solubilize ionic-bound pectin without degrading other cell wall components. • Reliable Uronic Acid Quantification: Employs a precise colorimetric method (e.g., meta-hydroxydiphenyl/sulfuric acid) for quantifying galacturonic acid, the primary component of pectin. • High-Throughput Plant Screening: The microplate format enables efficient comparison of pectin profiles across multiple plant samples or treatment conditions.

For a complete pectin profile, use this ionic-bound pectin assay kit alongside our water-soluble pectin assay kit and covalently-bound pectin assay kit. To study the enzymes responsible for pectin modification, Alta DiagnoTech offers polygalacturonase (PG) activity assay kits and pectin methylesterase (PME) activity assay kits. For general cell wall analysis, our cellulose and hemicellulose content assay kits are available.

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For research use only, not for clinical use.