Immunostaining Permeabilization Buffer (Nonionic Surfactant X-100)
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Immunostaining Permeabilization Buffer (Nonionic Surfactant X-100)

Cat.No: RERE-0058 Datasheet

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Product Name Immunostaining Permeabilization Buffer (Nonionic Surfactant X-100)
Catalog No. RERE-0058
Description This permeabilization buffer prepares cells, frozen sections and paraffin sections for in situ detection methods such as immunostaining. Its nonionic surfactant inserts into lipid membranes and opens pathways that expose antigens, nucleic acids and other targets, letting antibodies, probes and labels enter the cell interior so staining reaches full depth and intensity. The buffer arrives ready to use with strong permeabilizing action and no preparation steps.
Intended Use Permeabilization of cells, frozen sections and paraffin sections before immunostaining, immunofluorescence and immunohistochemistry detection.
Principle / Technology The nonionic surfactant solubilises targeted membrane regions, creating openings for antibody and probe entry while retaining cell morphology.
Performance Range / Specifications Strong permeabilizing capacity with ready to use convenience.
Specificity Exposes intracellular targets while preserving overall cell structure.
Reaction Conditions / Protocol Incubate the fixed specimen with the buffer, wash thoroughly, then proceed to blocking and antibody incubation.
Storage Conditions 2 to 8 °C
Shelf Life 1 year
Package Specifications 100 mL; 500 mL
Product Form Ready to use liquid buffer
Key Features Strong and convenient permeabilization; ready to use format; broad specimen compatibility.
Regulatory / Compliance For research use.
Batch-to-Batch Consistency Lot controlled manufacture.

For research use only, not for clinical use.

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