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Immunostaining Blocking Buffer

Cat.No: RERE-0060 Datasheet

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Product Details Related Products
Product Name Immunostaining Blocking Buffer
Catalog No. RERE-0060
Description This blocking buffer closes the staining block before antibody incubation in immunofluorescence, immunohistochemistry and immunocytochemistry. By occupying sites that would otherwise capture antibodies nonspecifically, it reduces background binding of primary and secondary reagents, lowers overall background and lifts the signal to noise ratio of the final image. The formulation arrives ready to use with no additional preparation, keeping the staining workflow moving.
Intended Use Blocking of cell and tissue specimens before antibody incubation in immunofluorescence, immunohistochemistry and immunocytochemistry.
Principle / Technology Blocking agents saturate nonspecific binding sites so antibodies bind only their intended targets.
Performance Range / Specifications Lower background and improved signal to noise ratio.
Specificity Suppresses nonspecific binding of both primary and secondary antibodies.
Reaction Conditions / Protocol Incubate the specimen with the buffer after permeabilization, then proceed directly to primary antibody incubation without washing.
Storage Conditions 2 to 8 °C
Shelf Life 6 months
Package Specifications 100 mL
Product Form Ready to use liquid buffer
Key Features Ready to use with no preparation; broad compatibility across IF, IHC and ICC; clean background.
Regulatory / Compliance For research use.
Batch-to-Batch Consistency Lot controlled manufacture.

For research use only, not for clinical use.

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