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| Product Name | Immunoprecipitation Wash Buffer, High Stringency, 10× |
| Catalog No. | ATR-B-YJL-0190 |
| Description | A high-stringency wash buffer concentrate for immunoprecipitation and co-immunoprecipitation experiments where reducing non-specific binding is critical. The formulation contains elevated salt and detergent concentrations to disrupt weak electrostatic and hydrophobic interactions while preserving specific antibody-antigen binding. The 10× concentrate format enables custom dilution to achieve varying stringency levels. |
| Intended Use | Removal of non-specifically bound proteins from immunoprecipitation complexes with tunable washing stringency. |
| Principle / Technology | Ionic and detergent-mediated disruption of non-specific protein interactions |
| Detection Method | Western blot or mass spectrometry analysis of washed immunoprecipitates |
| Sample Type | Protein A/G agarose or magnetic bead immunoprecipitation pellets |
| Performance Range / Specifications | 1× working solution: 50 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.2% NP-40, 0.1% SDS, 1 mM EDTA |
| Sensitivity / LOD | Reduces background to negligible levels as assessed by silver stain of wash eluate |
| Specificity | High salt and dual-detergent formulation removes hydrophobic and charge-based non-specific interactors simultaneously |
| Reaction Conditions / Protocol | Add 500 µL 1× buffer to bead pellet; vortex briefly; centrifuge; repeat for total 3–5 washes |
| Components / Formulation | 10× high stringency wash buffer concentrate |
| Storage Conditions | 2–8 °C; SDS may precipitate at 4 °C but redissolves upon warming to room temperature |
| Shelf Life | 18 months at 2–8 °C |
| Package Specifications | 100 mL concentrate |
| Product Form | Liquid concentrate |
| Quality Control | Each lot tested in a co-IP experiment using anti-FLAG beads on FLAG-tagged and untagged cell lysates; background protein carryover ≤5% |
| Key Features | Tunable dilution of 10× concentrate allows users to adjust stringency by varying the final salt and detergent concentration |
| Purity | ≥99%; DNase/RNase free; sterile-filtered 0.22 µm |
| Concentration | As specified on product label |
| Activity / Unit Definition | pH buffering capacity within ±0.05 pH units of stated value |
| Molecular Weight | Not applicable — inorganic/organic buffer salts |
| Source / Origin | Synthetic, prepared from analytical grade reagents |
| pH Range / Optimal pH | As stated in product specification |
| Shipping Conditions | Ambient temperature; protect from extreme heat |
| Expiration Date / Stability | 12–24 months at recommended storage temperature |
| Regulatory / Compliance | For research use and IVD manufacturing; ISO 13485 quality system |
| Compatibility | Compatible with standard biochemical and cell biology applications |
| Recommended Buffer System | Use as directed; compatible with biological assay systems |
| Application Notes / Precautions | Avoid contamination; use aseptic technique for sterile products; verify pH before critical applications |
| Batch-to-Batch Consistency | pH within ±0.05 units; conductivity within ±5% of reference lot |
For research use only, not for clinical use.
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