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| Product Name | IDA-Ni, IDA-Co and NTA-Ni His-Tag Protein Purification Magnetic Beads |
| Catalog No. | IRMGM-0055 |
| Description | Alta DiagnoTech magnetic agarose affinity media provide nickel- and cobalt-chelate options for rapid purification of soluble, intracellular, secreted or denatured His-tagged proteins. |
| Intended Use | One-step magnetic affinity purification of histidine-tagged proteins for research and process-development workflows. |
| Principle / Technology | Immobilized metal-affinity capture on magnetic agarose beads bearing IDA-nickel, IDA-cobalt or NTA-nickel chelates. |
| Detection Method | Magnetic separation followed by protein elution |
| Sample Type | Crude samples from bacterial, yeast, insect and mammalian-cell expression systems |
| Performance Range / Specifications | A complete purification can be finished within 1 hour. One-step target purity can exceed 95%; for products above 90% purity, typical recovery is 70-80%. Parallel-process standard deviations for target purity and yield are below 5%. |
| Specificity | Histidine-tagged soluble, intracellular, secreted and denatured proteins |
| Reaction Conditions / Protocol | Binding is favored at pH 7.4-8.5. Low imidazole levels may be used during binding and washing, followed by a higher imidazole concentration for elution. |
| Package Specifications | IDA-Ni and IDA-Co beads: 5 mL, 2 × 50 mL or 4 × 250 mL at 10% v/v and 30-150 µm; each chemistry is also available as a 10-reaction, 5 mL kit. NTA-Ni beads: 5 mL, 10 mL or 2 × 50 mL at 25% v/v and 10-30 µm. |
| Product Form | Magnetic agarose bead suspension or purification kit |
| Key Features | Direct capture from crude material; no column packing or flow-rate control; concentration and elution volume can be adjusted; suitable for parallel, high-throughput and scale-up processing. |
| Purity | One-step target-protein purity can exceed 95%; IDA-Ni and IDA-Co reference values are approximately 90% and 95%, respectively. |
| Concentration | IDA-Ni and IDA-Co: 10% v/v bead suspension; NTA-Ni: 25% v/v bead suspension |
| Compatibility | Soluble and denatured proteins expressed in bacterial, yeast, insect or mammalian cells |
| Recommended Buffer System | Imidazole-based binding, wash and elution buffers; pH 7.4-8.5 for binding |
| Application Notes / Precautions | Avoid EDTA and reducing agents in binding samples and buffers. For difficult elution, buffer optimization may include additional salt, detergent or denaturant; low-pH elution can strip metal ions and may require bead regeneration. |
| Batch-to-Batch Consistency | Parallel target-protein purity and yield standard deviations are below 5%. |
| Product Type | Immobilized metal-affinity magnetic agarose beads |
| Function / Efficacy | Capture, wash and elute His-tagged target proteins by magnetic separation. |
| Analytes / Targets | His-tagged proteins |
| Matrix | Magnetic agarose |
| Particle Size | IDA-Ni and IDA-Co: 30-150 µm; NTA-Ni: 10-30 µm |
| Ligand / Bonded Phase | IDA-Ni, IDA-Co or NTA-Ni metal-chelate affinity ligand |
| Dynamic Binding Capacity | Reference capacities: IDA-Ni approximately 40 mg/mL gel; IDA-Co approximately 25 mg/mL gel. A 10% suspension can bind approximately 3-4 mg protein per mL for high-affinity proteins. |
| Regeneration Conditions | The beads retained useful binding performance and purity after up to six regeneration cycles in the cited evaluation. |
For research use only, not for clinical use.
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