| Product Name | Human α-Smooth Muscle Actin (α-SMA) ELISA Kit |
| Catalog No. | DDCD-QCY-0007 |
| Description | This kit employs a sandwich ELISA method to quantitatively detect ACTA2 levels in serum, urine, and tissue homogenate samples. ACTA2 serves as a critical target, playing a pivotal role in maintaining cellular structure and motility. Research indicates its abnormal expression correlates with various diseases, including vascular disorders and tumors. Mechanistic studies focus on its functions in cellular signaling and tissue remodeling, offering novel approaches for disease diagnosis and treatment. |
| Application | This kit is suitable for research fields including liver/lung fibrosis model studies, tumor microenvironment analysis, and atherosclerosis mechanism exploration. It enables precise detection of dynamic changes in α-SMA across multiple biological samples, providing a reliable tool for fundamental research into extracellular matrix remodeling, pathological fibrosis progression, and mesenchymal cell activation. |
| Species | Homo sapiens (Human) |
| Assay Type | Quantitative sandwich |
| Samples | Serum, urine, tissue homogenates |
| Sample Volume | 50-100 uL |
| Detection Wavelength | 450 nm |
| Detection Range | 3.12-200 ng/mL |
| Sensitivity | 0.78 ng/mL |
| Test Time | 1-5 h |
| Composition of the Kit | A micro ELISA plate Two vials lyophilized standard One vial biotin-labled α-SMA antibody One vial HRP-avidin One vial biotin-antibody diluent One vial HRP-avidin diluent (15 mL/bottle) One vial sample diluent (50 mL/bottle) One vial wash buffer (25 x concentrate) One vial TMB substrate (10 mL/bottle) One vial stop solution (10 mL/bottle) Four adhesive strips (96 wells) An instruction manual |
| Self-provided Materials | Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set to 540 nm or 570 nm. Incubator providing stable culture conditions within a temperature range of 37°C ± 5°C. Centrifuge and vortex mixer. Absorbent paper for drying microplate wells. 50–300 μL multichannel pipette and pipette tips. Deionized or distilled water. Timer. Test tubes for dilution. |
| Storage | Store at 2-8°C. |
For research use only, not for clinical use.
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