| Product Name | Human Angiotensin-Converting Enzyme 2 (ACE2) ELISA Kit |
| Catalog No. | DDCD-QCY-0004 |
| Description | This kit employs a sandwich ELISA method to quantitatively detect ACE2 levels in serum, plasma, and tissue culture supernatant samples. ACE2 (angiotensin-converting enzyme 2) is a membrane protein located on the surface of human cells. As the primary receptor for the SARS-CoV-2 virus, its binding to the virus's surface S protein is crucial for viral entry into cells. Research indicates that ACE2 plays a significant role in SARS-CoV-2 infection. Its dynamic expression changes may be associated with the pathogenesis of COVID-19. Furthermore, ACE2 and key molecules in its signaling pathway are crucial for the development and repair of pulmonary inflammation. |
| Application | This kit can be applied in scientific research scenarios such as cardiovascular disease models, exploration of viral infection mechanisms, and validation of drug targets. For example, it can be used to study viral entry mechanisms by detecting dynamic changes in ACE2 levels in cell culture supernatants, or to analyze differences in ACE2 expression levels in biological fluid samples under various conditions. |
| Species | Homo sapiens (Human) |
| Assay Type | Quantitative sandwich |
| Samples | Serum, plasma, cell culture supernates |
| Sample Volume | 50-100 uL |
| Detection Wavelength | 450 nm |
| Detection Range | 0.156-10 ng/mL |
| Sensitivity | 0.039 ng/mL |
| Test Time | 1-5 h |
| Composition of the Kit | Micro ELISA plate Two vials lyophilized standard One vial biotin-labeled ACE2 antibody (100 x concentrate) One vial HRP-avidin (100 x concentrate) (120 μL/bottle) One vial biotin-antibody diluent (15 mL/bottle) One vial HRP-avidin diluent (15 mL/bottle) One vial Sample diluent (50 mL/bottle) One vial wash buffer (25 x concentrate) (20 mL/bottle) One vial TMB substrate (10 mL/bottle) One vial stop solution (10 mL/bottle) Four adhesive strips (96 wells) An instruction manual |
| Self-provided Materials | Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set to 540 nm or 570 nm. Incubator providing stable culture conditions within a temperature range of 37°C ± 5°C. Centrifuge and vortex mixer. Absorbent paper for drying microplate wells. 50–300 μL multichannel pipette and pipette tips. Deionized or distilled water. Timer. Test tubes for dilution. |
| Storage | Store at 2-8°C. |
For research use only, not for clinical use.
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