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| Product Name | Human Mast Cell Chymase ELISA Kit |
| Catalog No. | DDCD-QCY-0054 |
| Description | This kit is a sandwich ELISA assay designed for the quantitative detection of CMA1 levels in serum, tissue culture supernatant, and tissue homogenate samples. CMA1, also known as chymotrypsin/CMA1 protein, is a protease secreted by mast cells that participates in vasoactive peptide generation, extracellular matrix degradation, and glandular secretion regulation. |
| Application | This kit is suitable for research applications including the investigation of mast cell activation mechanisms, the establishment of inflammation-related disease models, and the evaluation of drug intervention efficacy. It provides reliable data support for immunology, pathology, and pharmacology research. |
| Species | Homo sapiens (Human) |
| Assay Type | Quantitative sandwich |
| Samples | Serum, cell culture supernates, tissue homogenates |
| Sample Volume | 50-100 uL |
| Detection Wavelength | 450 nm |
| Detection Range | 0.156-10 ng/mL |
| Sensitivity | 0.039 ng/mL |
| Test Time | 1-5 h |
| Composition of the Kit | A micro ELISA plate Two vials lyophilized standard One vial biotin-labeled mast cell chymase antibody One vial HRP-avidin One vial biotin-antibody diluent One vial HRP-avidin diluent One vial sample diluent One vial wash buffer One vial TMB substrate One vial stop solution Four adhesive strips An instruction manual |
| Self-provided Materials | Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set to 540 nm or 570 nm. Incubator providing stable culture conditions within a temperature range of 37°C ± 5°C. Centrifuge and vortex mixer. Absorbent paper for drying microplate wells. 50–300 μL multichannel pipette and pipette tips. Deionized or distilled water. Timer. Test tubes for dilution. |
| Storage | Store at 2-8°C. |
For research use only, not for clinical use.
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