| Product Name | Human Leukocyte Antigen (HLA) Gene DNA Typing Assay Kit (SYBR Green qPCR) |
| Catalog No. | BTRTK-HMM-0025 |
| Description | This kit is used to genotype human genomic DNA samples for the identification of human leukocyte antigens (HLA), to find appropriate donors in organ transplantation, and to avoid adverse reactions in blood and platelet transfusion. |
| Applications | This kit can be used to detect a total of 86 HLA class I and II loci, including: HLA-A (22 types), HLA-B (25 types), HLA-C (16 types), HLA-DRB1 (16 types), HLA-DQB1 (7 types). |
| Sample Type | Whole blood ≥1 mL (sodium citrate or EDTA anticoagulation); DNA ≥150 μL (concentration 20-40 ng/μL, A260/A280 value 1.6-2.0); Amniotic fluid: ≥4 mL (more than 16 weeks), the above three kinds of specimens were stored at 2-8°C for 2 weeks, and ≤-20°C for 1 year. Oral mucosa swabs (2, respectively, with brushing force to collect oral mucosa cells from both cheeks, stored at room temperature for 1 week). |
| Detection Methods | SYBR Green qPCR |
| Estimated Measurement Time | 1 hour and 41 minutes (one-step (10min) operation, amplification products do not need to be transferred for spiking, avoiding laboratory and aerosol contamination). |
| Shelf Life | 18 months |
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