| Product Name | Human KIR and HLA-Bw/C Genotyping Assay Kit (SYBR Green qPCR) |
| Catalog No. | BTRTK-HMM-0030 |
| Description | KIR and HLA ligand can guide NK cell screening in NK cell therapy. Adverse reactions such as acute rejection (AR) or viral infections are reduced by testing the fit of recipient KIR and donor HLA ligand before solid organ transplantation. Detection of donor KIR and recipient HLA ligand typing before hematology-related transplantation (hematopoietic stem cell transplantation, umbilical cord blood transplantation) can be used as a basis for donor selection. |
| Applications | This kit can be used to detect 20 KIR genes and 4 HLA ligands: 2DL1, 2DL2, 2DL3, 2DL4, 2DL5A*00101-040, 2DL5B*0020101-00204/004-00601/ 00603-041, 2DL5B*003/00602, 2DS1, 2DS2, 2DS3, 2DS4*00101-00106/01101-01102/014-017 /019-022 (FUL), 2DS4 (DEL) *0030101-010/012-013/018 (DEL), 2DS5, 3DL1, 3DL2, 3DL3, 3DS1, 3DP1, 3DP1 *001-002/004/007/0090101-00902/011-012(FUL),3DP10030101-0030402/005-006/008/01001-01002/013-052(DEL), Bw4, Bw6, HLA-C Group 1 (C1), and HLA-C Group 2 (C2). |
| Sample Type | Whole blood ≥1 mL (sodium citrate or EDTA anticoagulation); DNA ≥50 μL (concentration 20-40 ng/μL, A260/A280 value 1.6-2.0); Amniotic fluid: ≥4 mL (more than 16 weeks), the above three kinds of specimens were stored at 2-8°C for 2 weeks, and ≤-20°C for 1 year. Oral mucosa swabs (2, respectively, with brushing force to collect oral mucosa cells from both cheeks, stored at room temperature for 1 week). |
| Detection Methods | SYBR Green qPCR |
| Estimated Measurement Time | 1 hour and 41 minutes (one-step (10min) operation, amplification products do not need to be transferred for spiking, avoiding laboratory and aerosol contamination). |
| Shelf Life | 18 months |
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