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Cat.No: DB-1612 Datasheet Instruction for Use
Specification Quantities
Price $1402.95–2338.25
| Product Name | Human Hantavirus (HV) Antibody (IgG) ELISA Kit |
| Catalog No. | DB-1612 |
| Description | The kit employs an indirect enzyme-linked immunosorbent assay (ELISA). Samples, negative controls, and positive controls are sequentially added to pre-coated microplates containing Hantavirus IgG (HVIgG) capture antibodies. HRP-labeled detection antibodies are then added, followed by incubation and thorough washing. Color development is achieved using the substrate TMB, which is converted to blue under the catalysis of horseradish peroxidase and further converted to yellow under acidic conditions. The intensity of the color is directly proportional to the concentration of Hantavirus IgG (HVIgG) in the sample. Absorbance (OD value) is measured at a wavelength of 450 nm using a microplate reader to determine positive or negative results. |
| Assay Method | ELISA |
| Reagent Preparation | Before use, all components must be allowed to stand at room temperature for at least 60 minutes to ensure they are fully warmed to room temperature. Concentrated Wash Buffer: When removed from the refrigerator, the concentrated wash buffer may form crystals; this is normal. Heat it in a water bath until the crystals are completely dissolved. Dilute the concentrated wash buffer with distilled water at a ratio of 1:20, i.e., add 19 parts distilled water to 1 part concentrated wash buffer. |
| Test Procedure | All reagents and components should be brought to room temperature first. It is recommended to duplicate the standard, control, and sample wells. Remove the required strip from the foil pouch; seal the remaining strips in a resealable bag and return them to the refrigerator. Set up the standard and sample wells. Add 50 μL of each different concentration of standard to the standard wells; add 50 μL of the sample to each sample well; leave the blank wells empty. Except for the blank wells, add 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each standard and sample well. Cover the wells with a sealing film and incubate in a 37°C water bath or incubator for 60 minutes. Discard the liquid, blot dry with absorbent paper, fill each well with washing solution (350 μL), let stand for 1 minute, remove the washing solution by shaking, and blot dry with absorbent paper. Repeat this washing procedure 5 times (a plate washer may also be used). Add 50 μL each of Substrates A and B to each well, and incubate at 37°C in the dark for 15 minutes. Add 50 μL of stop solution to each well, and measure the OD values of each well at a wavelength of 450 nm within 15 minutes. |
| Results | Negative control OD value: less than 0.2. Positive control OD value: greater than 0.8. Positive Cut-Off Value: Negative control OD value + 0.25. If the sample OD value exceeds this threshold, the result is considered positive; otherwise, it is negative. |
For research use only, not for clinical use.
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