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| Product Name | Human Ang I (Angiotensin-1) ELISA Kit |
| Catalog No. | HTK-YJL-0007 |
| Synonyms | Ang I ELISA Kit, Angiotensin-I ELISA Kit |
| Species | Human |
| UniProt ID | P01019 |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 125-8000 pg/ml |
| Sensitivity | 75 pg/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with Ang I, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Angiotensin-1 (Ang I) is a key peptide intermediate in the renin-angiotensin system (RAS), a critical regulatory pathway for mammalian blood pressure, fluid balance, and cardiovascular homeostasis. Its biological origin and function are tightly linked to the body’s adaptive responses to changes in renal perfusion, electrolyte levels, and vascular tone—making it a vital target for research in cardiovascular physiology, nephrology, and related disease mechanisms.
Biosynthesis and Conversion Pathway: Ang I is generated exclusively through the enzymatic action of renin, an aspartic protease produced by juxtaglomerular cells in the kidneys. Renin cleaves the peptide bond between leucine (Leu) and valine (Val) residues in angiotensinogen—a glycoprotein synthesized in the liver—resulting in a 10-amino acid peptide (des-asp angiotensin I). Notably, Ang I itself exhibits no direct biological activity; its primary role is to serve as a precursor for Angiotensin II (Ang II), the biologically active effector of the RAS.
Key Role in RAS Signaling: Ang I is converted to Ang II via the removal of two terminal amino acids by Angiotensin-Converting Enzyme (ACE), an enzyme most densely expressed in pulmonary capillaries (due to the high density of capillary beds in the lungs, though ACE is present throughout the body). Ang II then acts as a multifunctional hormone: it induces vasoconstriction to elevate blood pressure, stimulates aldosterone secretion from the adrenal cortex (promoting sodium and water retention), and modulates renal sodium reabsorption—all critical for maintaining circulatory homeostasis.
Research Relevance: Given its position as a central intermediate in the RAS, Ang I is widely studied to elucidate the pathogenesis of hypertension, chronic kidney disease (CKD), heart failure, and other cardiovascular disorders. For example, dysregulation of renin activity (and subsequent Ang I production) is associated with primary hypertension, while altered Ang I/Ang II conversion dynamics are linked to renal dysfunction and cardiovascular remodeling. Accurate quantification of Ang I in biological samples (e.g., serum, plasma, tissue lysates) is therefore essential for understanding RAS activation in both physiological and pathological states.
Broad Sample Compatibility: Designed to detect Ang I in multiple human biological matrices, including serum, plasma, cell culture supernatants, cell lysates, and tissue homogenates—eliminating the need for specialized kits for different sample types and supporting diverse research workflows.
Reliable Detection Chemistry: Utilizes a competitive ELISA format with pre-coated Ang I antigen, ensuring specific binding between the target analyte and biotinylated anti-Ang I antibodies. This design minimizes non-specific binding and cross-reactivity, delivering consistent results across experiments.
Clear Detection Wavelength: Optimized for measurement at 450 nm (a standard wavelength for microplate readers), enabling easy integration with most laboratory equipment and reducing the need for specialized detection tools.
Controlled Reaction Timing: Features a total reaction duration of 2 hours, balancing efficiency (supporting high-throughput experiments) and accuracy (ensuring sufficient time for antigen-antibody binding and enzymatic reactions).
Stable Storage Conditions: Requires only 2-8°C storage (no cryopreservation needed) for unopened components, simplifying inventory management and reducing the risk of performance loss due to improper freezing/thawing.
High Specificity: Undergoes rigorous validation to ensure exclusive binding to human Ang I—no significant cross-reactivity with Ang I analogues (e.g., Ang II, angiotensinogen fragments) or other related peptides, guaranteeing that detected signals reflect true Ang I concentrations.
Optimal Detection Range: Covers a range of 125–8000 pg/ml, which aligns with the physiological concentration of Ang I in human biological samples (e.g., serum Ang I levels typically fall within 200–1500 pg/ml in healthy individuals). This range eliminates the need for excessive sample dilution (reducing error) and accommodates samples from pathological states with elevated Ang I.
Sensitive Quantification: Achieves a sensitivity of 75 pg/ml, enabling detection of low-level Ang I in samples with minimal target expression (e.g., cell culture supernatants or early-stage disease models) that might be undetectable with less sensitive kits.
Research-Grade Performance: Manufactured to meet strict quality standards, with pre-validated intra-assay and inter-assay coefficient of variation (CV) to ensure reproducibility—critical for generating reliable data for publications or long-term studies.
User-Friendly Protocol: Includes all necessary components (pre-coated plate, standards, antibodies, substrates) and a straightforward procedure, reducing hands-on time and lowering the learning curve for new users.
For research use only, not for clinical use.
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