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| Product Name | High-Yield One-Step RT-PCR Kit with Loading Dye |
| Catalog No. | MDKPK-0040 |
| Description | A high-yield one-step endpoint RT-PCR kit with integrated loading dye and a genomic DNA removal module. |
| Intended Use | Virus detection, gene-expression analysis and endpoint RT-PCR. |
| Principle / Technology | Reverse transcription and DNA amplification occur in one tube using an optimized enzyme blend and buffer. A separate genomic DNA removal component reduces residual DNA before amplification. |
| Detection Method | Endpoint RT-PCR followed by direct agarose-gel electrophoresis. |
| Sample Type | Total RNA, viral RNA and other compatible RNA templates. |
| Performance Range / Specifications | 50 reactions; supports multiplex amplification and RNA-derived amplicons up to approximately 10 kb; PCR products can be loaded directly onto a gel. |
| Sensitivity / LOD | Reported detection reached 0.1 pg total RNA. |
| Specificity | Optimized reverse transcriptase, DNA polymerase and buffer improve yield and amplification specificity. |
| Reaction Conditions / Protocol | Use the genomic DNA removal component when required, then combine RNA, gene-specific primers and the one-step RT-PCR reagent. Load the completed product directly for electrophoresis. |
| Components / Formulation | One-step RT-PCR premix with loading dye, reverse-transcription and DNA polymerase activities, plus a 5× genomic DNA removal component. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 50 reactions. |
| Product Form | One-tube RT-PCR kit with loading dye and genomic DNA removal module. |
| Quality Control | Amplification yield, 0.1 pg RNA sensitivity, long-fragment performance and multiplex compatibility are evaluated. |
| Key Features | High yield; 0.1 pg RNA sensitivity; amplicons up to approximately 10 kb; multiplex support; genomic DNA removal; direct gel loading. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for conventional thermal cyclers and agarose-gel endpoint analysis. |
| Application Notes / Precautions | Use gene-specific primers. Optimize extension time for long amplicons and use high-quality intact RNA. |
For research use only, not for clinical use.
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