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| Product Name | High-Yield One-Step RT-PCR Kit |
| Catalog No. | MDKPK-0041 |
| Description | A high-yield one-step RT-PCR kit for endpoint detection of RNA targets, with a dedicated genomic DNA removal module. |
| Intended Use | Virus detection, gene-expression analysis and endpoint RT-PCR. |
| Principle / Technology | Reverse transcription and PCR take place sequentially in one tube using an optimized reverse transcriptase, DNA polymerase and buffer formulation. |
| Detection Method | Endpoint RT-PCR with agarose-gel electrophoresis or other downstream amplicon analysis. |
| Sample Type | Total RNA, viral RNA and other compatible RNA templates. |
| Performance Range / Specifications | 50 reactions; supports multiplex amplification and RNA-derived amplicons up to approximately 10 kb. |
| Sensitivity / LOD | Reported detection reached 0.1 pg total RNA. |
| Specificity | Optimized enzyme and buffer chemistry improve product yield and specific amplification. |
| Reaction Conditions / Protocol | Treat RNA with the genomic DNA removal component when needed, then combine RNA and gene-specific primers with the one-step RT-PCR reagent and run an endpoint program. |
| Components / Formulation | One-step RT-PCR reagent containing reverse-transcription and DNA polymerase functions, plus a 5× genomic DNA removal component. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 50 reactions. |
| Product Form | One-tube RT-PCR kit with genomic DNA removal module. |
| Quality Control | Yield, low-input sensitivity, long-fragment amplification and multiplex performance are evaluated. |
| Key Features | High yield; 0.1 pg RNA sensitivity; long-fragment capability; multiplex support; genomic DNA removal. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for conventional thermal cyclers and endpoint amplicon analysis. |
| Application Notes / Precautions | Use gene-specific primers and adjust extension time according to target length. |
For research use only, not for clinical use.
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