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| Product Name | High-Sensitivity One-Step RT-qPCR Probe Kit with Carryover Control |
| Catalog No. | MDKPK-0031 |
| Description | A probe-based one-step RT-qPCR kit integrating reverse transcription, RNase protection, hot-start PCR and dUTP/thermolabile UDG carryover control in a streamlined workflow. |
| Intended Use | Virus detection, pathogen testing, fusion-gene analysis, combined DNA/RNA testing, gene-expression analysis and residue testing. |
| Principle / Technology | Reverse transcription and qPCR occur in one tube using a reverse transcriptase, RNase inhibitor and antibody-regulated Taq polymerase. Thermolabile UDG removes uracil-containing carryover DNA and becomes inactive during reverse transcription. |
| Detection Method | TaqMan-type and other fluorescent probe-based real-time RT-qPCR. |
| Sample Type | RNA templates, including viral RNA, and compatible DNA/RNA assay templates. |
| Performance Range / Specifications | 100, 1,000 or 5,000 reactions at 30 µL per reaction; supports singleplex, multiplex and fast cycling programs; provides balanced amplification over high and low template concentrations. |
| Specificity | Hot-start polymerase control and optimized buffer chemistry improve target specificity in singleplex and multiplex assays. |
| Reaction Conditions / Protocol | Prepare the reaction with the supplied 5× reaction mix, enzyme mix, assay-specific primers/probes and template. Select the appropriate ROX reference dye only when required by the instrument. |
| Components / Formulation | RNase-free water; 5× one-step reaction mix containing dNTP/dUTP and magnesium; enzyme mix containing reverse transcriptase, RNase inhibitor, thermolabile UDG and hot-start Taq polymerase; two ROX reference dye options. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions; 1,000 reactions; 5,000 reactions. |
| Product Form | Two-component probe-based one-step RT-qPCR kit with reference dyes. |
| Quality Control | Sensitivity, multiplex performance, fast-program compatibility, storage stress and freeze–thaw stability are evaluated. |
| Key Features | High sensitivity; multiplex capability; rapid cycling compatibility; balanced high/low-template amplification; strong storage stability; carryover contamination control. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | Reported performance remained stable after 10, 30 and 50 freeze–thaw cycles and up to 7 days at 37°C. |
| Compatibility | Compatible with common real-time PCR platforms; ROX selection depends on instrument requirements. |
| Application Notes / Precautions | Use ROX Reference Dye 1 or 2 only for instruments that require passive reference normalization; many Roche and Bio-Rad systems do not require ROX. |
For research use only, not for clinical use.
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