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| Product Name | High-Sensitivity One-Step RT-qPCR Probe Kit |
| Catalog No. | MDKPK-0034 |
| Description | A sensitive one-step probe RT-qPCR kit for quantitative detection of RNA targets without a separate cDNA synthesis step. |
| Intended Use | Virus detection, pathogen testing and gene-expression analysis. |
| Principle / Technology | Reverse transcription and hot-start probe qPCR are performed sequentially in the same tube using an optimized enzyme blend and buffer system. |
| Detection Method | Fluorescent probe-based real-time RT-qPCR. |
| Sample Type | Total RNA, viral RNA and other compatible RNA templates. |
| Performance Range / Specifications | 250 reactions at 20 µL per reaction; reported linear response over five orders of magnitude with cellular RNA and seven orders of magnitude with in vitro-transcribed RNA; supports multiplex probe detection. |
| Sensitivity / LOD | Reported detection reached 0.1 pg total RNA or approximately 10 RNA copies. |
| Specificity | The optimized enzyme system supports specific probe detection in singleplex and duplex reactions. |
| Reaction Conditions / Protocol | Combine assay-specific primers/probes, RNA template and supplied reaction components in a 20 µL reaction and run a compatible one-step RT-qPCR program. |
| Components / Formulation | One-step probe RT-qPCR reaction mix and enzyme blend for reverse transcription and hot-start DNA amplification. |
| Package Specifications | 250 reactions (20 µL per reaction). |
| Product Form | Two-component one-step RT-qPCR probe kit. |
| Quality Control | Sensitivity, linearity and multiplex probe performance are evaluated. |
| Key Features | One-tube workflow; low-copy sensitivity; broad linear range; multiplex probe compatibility. |
| Compatibility | Suitable for common probe-based real-time PCR platforms. |
| Application Notes / Precautions | Use gene-specific primers and fluorescence probes and include no-template and positive controls. |
For research use only, not for clinical use.
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