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| Product Name | High-Sensitivity Fast One-Step RT-qPCR Probe Kit |
| Catalog No. | MDKPK-0035 |
| Description | A high-sensitivity one-step probe RT-qPCR kit supporting rapid cycling, multiplex detection and thermolabile UDG carryover control. |
| Intended Use | Virus detection, pathogen testing and gene-expression analysis. |
| Principle / Technology | An optimized reverse transcriptase and antibody-regulated hot-start Taq polymerase provide efficient amplification, while thermolabile UDG degrades uracil-containing carryover DNA before becoming inactive during reverse transcription. |
| Detection Method | Fluorescent probe-based real-time RT-qPCR. |
| Sample Type | RNA templates, including viral and cellular RNA. |
| Performance Range / Specifications | 100 or 5,000 reactions at 30 µL per reaction; compatible with fast cycling and multiplex probe assays. |
| Sensitivity / LOD | Designed for highly sensitive amplification of low-level RNA targets. |
| Specificity | Hot-start regulation and carryover-control chemistry reduce nonspecific amplification and contamination risk. |
| Reaction Conditions / Protocol | Set up a 30 µL one-step reaction with assay-specific primers/probes, template and the supplied reaction components. |
| Components / Formulation | 2× one-step reaction mix containing optimized buffer and dNTP/dUTP, plus an enzyme blend with reverse transcriptase, thermolabile UDG and hot-start Taq polymerase. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions; 5,000 reactions (30 µL per reaction). |
| Product Form | Two-component one-step RT-qPCR probe kit. |
| Quality Control | Amplification sensitivity, fast-program compatibility, multiplex performance and carryover-control efficiency are evaluated. |
| Key Features | High sensitivity; fast cycling; multiplex support; thermolabile UDG contamination control. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Suitable for probe-based real-time PCR instruments. |
| Application Notes / Precautions | Use an assay program compatible with thermolabile UDG and one-step reverse transcription. |
For research use only, not for clinical use.
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