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| Product Name | HEPES hemisodium salt biological buffer for cell culture and protein crystallization, CAS 103404-87-1, purity >99% |
| Catalog No. | ATR-B-YJL-0218 |
| Description | HEPES hemisodium salt (4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt) is a buffer widely used in the biomedical field. It maintains a stable pH in biological experiments, providing a consistent internal environment that protects the activity and stability of biological samples. Its key characteristics include high solubility, strong buffering capacity, and low cytotoxicity. With an effective buffering range of approximately pH 6.8–8.2, it reliably stabilizes pH, while its high solubility allows use in a variety of solvent systems. The low cytotoxicity is particularly valuable for biological samples, preserving their activity and preventing errors caused by cell damage during experiments. |
| Intended Use | Used as a buffer in biomedical research to maintain stable pH in cell culture media, protein crystallography, biomolecular detection, and immunoassay applications. |
| Package Specifications | 25 kg/drum |
| Key Features | High solubility, enabling use in a wide range of solvent systems Strong buffering capacity with an effective range of approximately pH 6.8–8.2 Low cytotoxicity, protecting the biological activity of samples and reducing experimental error from cell damage |
| Purity | >99% |
| pH Range / Optimal pH | Effective buffering range approximately pH 6.8–8.2 |
| Application Notes / Precautions | Cell culture: can be added to culture media as a buffer to provide a stable environment for cell growth Protein crystallography: serves as a crystallization buffer to support protein crystal formation Biomolecular detection and immunoassays: widely used to maintain stable assay conditions Precautions: select the appropriate pH according to the specific sample and experimental conditions, since different samples have different pH requirements; control the concentration and duration of use to avoid adverse effects on biological samples, as HEPES hemisodium salt has a degree of cytotoxicity |
| Alias | HEPES 0.5Na; HEPES hemisodium salt; 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium salt |
| CAS Number | 103404-87-1 |
For research use only, not for clinical use.
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