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| Product Name | HEPES buffer preparation reagent for laboratory buffer formulation, CAS 7365-45-9 |
| Catalog No. | ATR-B-YJL-0302 |
| Description | HEPES is a zwitterionic buffering reagent for preparing biologically compatible buffers, with a pKa of 7.55 at 25°C and an effective range of pH 6.8-8.2. To prepare a 1 M solution, dissolve 238.3 g in approximately 800 mL of purified water, adjust with NaOH to the required pH, typically 7.0-7.6, and bring the final volume to 1 L. The resulting buffer is suitable for cell culture, protein research and enzymology, with lower cellular toxicity and stronger pH stability than phosphate buffer. |
| Intended Use | Laboratory preparation of HEPES buffer for cell culture, protein research and enzyme experiments. |
| Principle / Technology | Zwitterionic buffering with a pKa of 7.55 at 25°C and an effective buffering range of pH 6.8-8.2. |
| Reaction Conditions / Protocol | For a 1 M HEPES solution, dissolve 238.3 g of powder in approximately 800 mL of purified water, adjust to the required pH, typically 7.0-7.6, with NaOH, and dilute to a final volume of 1 L. Filter-sterilize and store at 4°C. |
| Components / Formulation | Supplied as HEPES powder; the described 1 M buffer is prepared with purified water and pH-adjusted with sodium hydroxide. |
| Storage Conditions | After filter sterilization, store the prepared buffer at 4°C. Protect HEPES solutions from light. |
| Package Specifications | 25 kg per drum. |
| Product Form | White crystalline powder. |
| Quality Control | Purity >99%; supplied as a white crystalline powder. |
| Key Features | Effective buffering from pH 6.8-8.2; lower toxicity to cells than phosphate buffer; strong pH stability in culture systems. |
| Purity | >99% |
| Molecular Weight | 238.30 |
| pH Range / Optimal pH | Effective buffering range: pH 6.8-8.2; pKa 7.55 at 25°C. |
| Application Notes / Precautions | Do not use high-temperature, high-pressure sterilization; filter sterilization is recommended. Protect HEPES solutions from light to prevent degradation. |
| Molecular Formula | C8H18N2O4S |
| Appearance | White crystalline powder. |
| CAS Number | 7365-45-9 |
For research use only, not for clinical use.
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