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| Product Name | Hepes buffer for diagnostic kits, CAS 7365-45-9, purity ≥99% |
| Catalog No. | ATR-B-YJL-0239 |
| Description | HEPES, chemically named 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (CAS 7365-45-9), is a white crystalline powder supplied at a purity of no less than 99%. It provides effective buffering over the pH range of 6.8 to 8.2, making it one of the most widely used zwitterionic buffers in molecular biology and in vitro diagnostics. HEPES is commonly applied in the 3'-terminal labeling of RNA using T4 RNA ligase and serves as a key buffer component in biochemical diagnostic kits, DNA/RNA extraction kits, and PCR-based diagnostic kits. |
| Intended Use | 3'-terminal labeling of RNA with T4 RNA ligase; buffer component in biochemical diagnostic kits, DNA/RNA extraction kits, and PCR diagnostic kits. |
| Reaction Conditions / Protocol | To prepare 500 mL of a 1 M HEPES stock solution at pH 7.0, dissolve 119.15 g of HEPES in 400 mL of distilled water. Adjust the solution to the desired pH using 0.5-1 M aqueous NaOH, then bring the final volume to 500 mL with distilled water. Store the stock solution at 4°C. Buffer solutions of other concentrations and volumes can be prepared in the same manner by scaling the proportions accordingly. |
| Storage Conditions | Store prepared stock solutions at 4°C. |
| Package Specifications | 500 g per bottle; 25 kg per drum |
| Product Form | White crystalline powder |
| Purity | ≥99% |
| pH Range / Optimal pH | 6.8-8.2 |
| Application Notes / Precautions | HEPES can generate hydrogen peroxide upon exposure to light, which is toxic to cultured cells and other biologically active materials. Buffer preparation and handling involving HEPES should therefore be performed under light-protected conditions whenever possible. |
| Appearance | White crystalline powder |
| Alias | HEPES; 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid |
| CAS Number | 7365-45-9 |
For research use only, not for clinical use.
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