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| Product Name | HEPES buffer for CO2-independent cell culture and low-temperature enzyme studies, CAS 7365-45-9 |
| Catalog No. | ATR-B-YJL-0303 |
| Description | HEPES maintains stable medium pH during cell culture under open, CO2-free or low-CO2 conditions. Its low cytotoxicity and strong buffering across pH 6.8-8.2 support cell growth without requiring a CO2 atmosphere. Because its pKa changes relatively little at 4°C, it is also suitable for low-temperature enzymatic studies. |
| Intended Use | pH stabilization in cell culture media and low-temperature enzyme reaction systems. |
| Principle / Technology | Zwitterionic buffering maintains pH outside a CO2-controlled environment and provides stable performance at low temperature. |
| Reaction Conditions / Protocol | Typical working concentration in cell culture is 10-25 mM. Suitable for enzyme reaction systems maintained at 4°C. |
| Package Specifications | 25 kg per drum. |
| Product Form | White crystalline powder. |
| Quality Control | Purity >99%; supplied as a white crystalline powder. |
| Key Features | Low cytotoxicity; supports normal cell growth; strong buffering from pH 6.8-8.2; does not require a CO2 environment; limited pKa change at 4°C. |
| Purity | >99% |
| Molecular Weight | 238.30 |
| pH Range / Optimal pH | Most effective from pH 6.8-8.2. |
| Application Notes / Precautions | Use at 10-25 mM for cell culture. Suitable for open handling in CO2-free or low-CO2 environments. |
| Molecular Formula | C8H18N2O4S |
| Appearance | White crystalline powder. |
| CAS Number | 7365-45-9 |
For research use only, not for clinical use.
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