- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | HEPES Buffer, 1 M, pH 7.3 |
| Catalog No. | ATR-B-YJL-0167 |
| Description | A zwitterionic biological buffer providing robust pH control in the physiological range. HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is preferred for cell culture applications where CO2 incubation may cause pH drift. |
| Intended Use | Buffering cell culture media in ambient CO2 conditions, maintaining pH stability during imaging experiments, and serving as a buffer in biochemical assays requiring precise pH control. |
| Principle / Technology | Zwitterionic sulfonic acid buffer with pKa of 7.55 at 25°C; minimal metal ion chelation compared to phosphate buffers. |
| Detection Method | pH electrode |
| Sample Type | Mammalian cell cultures, fluorescence microscopy specimens, enzymatic assay mixtures |
| Performance Range / Specifications | pH 7.1–7.5 at working dilution, buffering range pH 6.8–8.2 |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Use at 10–25 mM final concentration; sterile filter for cell culture use. |
| Components / Formulation | HEPES free acid dissolved in ultrapure water, pH adjusted with sodium hydroxide |
| Storage Conditions | 15–30°C, protect from light |
| Shelf Life | 36 months |
| Package Specifications | 100 mL, 500 mL, 1 L |
| Product Form | Liquid concentrate |
| Quality Control | pH verification, cell culture tested for toxicity, endotoxin <0.05 EU/mL, sterility |
| Key Features | Cell culture tested; minimal CO2-dependent pH drift; low metal chelation; suitable for fluorescence microscopy |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
