- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
Cat.No: DB-1611 Datasheet Instruction for Use
Specification Quantities
Price $961.77–1602.95
| Product Name | Hantavirus PCR Test Kit |
| Catalog No. | DB-1611 |
| Description | Hantavirus is an RNA virus that primarily causes hemorrhagic fever with renal syndrome (HFRS). Rodents and other animals serve as the main sources of infection. The disease is characterized by fever, bleeding, congestion, hypotensive shock, and kidney damage, and is a significant zoonotic disease with a high case-fatality rate (overall mortality ranging from 6% to 15%). This product is a kit specifically designed to detect Hantavirus RNA, developed using probe-based qRT-PCR technology. |
| Kit Components | Probe-based qRT-PCR Buffer: 0.5 mL Probe-based qRT-PCR Enzyme Mix v2: 50 μL Fluorescent PCR Template Dilution Buffer: 1 mL Hantavirus qRT-PCR Primer-Probe Lyophilized Powder: 50 Tests Hantavirus qRT-PCR Positive Control (E7 copies/μL): 50 μL User Manual: 1 Copy |
| Assay Method | PCR |
| Reagent Preparation | Label 6 centrifuge tubes as 6, 5, 4, 3, 2, and 1. Add 45 µL of Fluorescent PCR Template Dilution Buffer to each tube using a filter tip. (Use filter tips for all subsequent steps). Add 5 µL of E7 copies/µL Positive Control (provided in the kit) to tube #6. Mix thoroughly for 1 minute. This yields the E6 copies/mL standard curve sample. Place on ice. Change tip. Add 5 µL of the E6 copies/µL positive control (diluted in the previous step) to tube #5. Mix thoroughly for 1 minute. This yields the E5 copies/mL standard curve sample. Place on ice. Change tip. Add 5 µL of the E5 copies/µL positive control (diluted in the previous step) to tube #4. Mix thoroughly for 1 minute. This yields the E4 copies/µL standard curve sample. Place on ice. Repeat the above steps until you obtain standard curve samples for all 6 dilutions. Place on ice. |
| Sample Preparation | If there are N samples, set up N+2 extractions. The extra two are for: PC (sample preparation positive control), NC (sample preparation negative control). Purify the sample DNA using your preferred method. This kit is compatible with most commercial DNA extraction kits on the market. Alternatively, you can purchase our company's extraction-free nucleic acid release agent. |
| Test Procedure | Tube Setup: For Quantitative Analysis (1 repeat): Label N+9 RT-PCR tubes. N+2 tubes: For the N+2 samples obtained in the previous step. 1 tube: For RT-PCR Negative Control (Water as template). 6 tubes: For the Standard Curve. For Qualitative Analysis (1 repeat): Label N+4 RT-PCR tubes. N+2 tubes: For the N+2 samples obtained in the previous step. 1 tube: For RT-PCR Negative Control (Water as template). 1 tube: For RT-PCR Positive Control (Directly use the diluted positive control from tube #4 in Step 6 as template). Run the RT-PCR. If the Sample Preparation Positive Control or PCR Positive Control (including standard curve samples) is Negative, the entire experiment is invalid. Discard data, re-prepare samples, re-run PCR, or contact the manufacturer. If the Sample Preparation Negative Control or PCR Negative Control is Positive, it indicates environmental contamination. The experiment is invalid. Discard data and contact the manufacturer. If both Negative and Positive controls are normal, the experiment is valid, and you can proceed to analysis. If Negative and Positive controls are normal, the experiment is valid. Proceed to subsequent analysis. Quantitative Analysis: Plot the standard curve using the log value of the positive control concentration as the X-axis and the Ct value as the Y-axis. Calculate the log value of the sample RNA concentration based on the sample's Ct value on the standard curve, then calculate the actual concentration. |
| Results | Negative Control Ct: Must have no reading, or be > 40. Positive Control: Must show logarithmic fluorescence increase, have a typical amplification curve, and Ct value < 40. Test Sample: If Ct has no reading, is > 40, or is = 40 → Negative. If Ct is < 40 → Positive. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
