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| Product Name | Glycine-HCl Buffer, 0.2 M, pH 2.2 |
| Catalog No. | ATR-B-YJL-0184 |
| Description | A strongly acidic glycine-hydrochloric acid buffer for antibody elution in affinity chromatography, low-pH virus inactivation, and pH conditioning in protein analytical methods. |
| Intended Use | Elution of antibodies from Protein A, Protein G, and antigen affinity columns; low-pH treatment for virus inactivation in bioprocessing; acidic conditioning in amino acid analysis. |
| Principle / Technology | Fully protonated glycine at low pH provides electrostatic shielding that disrupts antibody-antigen and antibody-Protein A/G binding interactions; hydrochloride serves as the acidic counterion. |
| Detection Method | pH electrode, UV absorbance monitoring at 280 nm for eluted protein |
| Sample Type | Affinity-captured antibodies, recombinant proteins, virus inactivation samples |
| Performance Range / Specifications | pH 2.0–2.4; elutes >90% of bound IgG from Protein A columns |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Apply 2–3 column volumes to affinity resin; collect fractions into 1 M Tris pH 9.0 for immediate neutralization; typical contact time 2–5 minutes. |
| Components / Formulation | Glycine in deionized water, pH adjusted to 2.2 with concentrated HCl |
| Storage Conditions | 15–30°C |
| Shelf Life | 12 months |
| Package Specifications | 100 mL, 500 mL, 1 L |
| Product Form | Liquid |
| Quality Control | pH verification, antibody elution efficiency test with standard IgG |
| Key Features | Standard antibody elution buffer; immediate neutralization required; validated for Protein A, G, and L resins; bioprocessing grade available |
For research use only, not for clinical use.
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