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| Product Name | Glycerol-Free One-Step RT-qPCR Probe Kit |
| Catalog No. | MDKPK-0045 |
| Description | A glycerol-free one-step probe RT-qPCR reagent designed for sensitive RNA amplification and development of lyophilized diagnostic formulations. |
| Intended Use | Virus detection, pathogen testing, fusion-gene analysis and gene-expression analysis. |
| Principle / Technology | A dedicated reverse transcriptase and hot-start Taq DNA polymerase operate in a lyophilization-compatible chemical buffer. A dUTP/UDG system removes uracil-containing carryover DNA before amplification. |
| Detection Method | Real-time fluorescent probe-based RT-qPCR. |
| Sample Type | RNA templates, including RNA viruses, and compatible crude samples such as stool, tissue and swab lysates. |
| Performance Range / Specifications | 200, 1,000 or 5,000 reactions; compatible with multiple freeze-drying processes and complex sample matrices. |
| Specificity | Hot-start polymerase control and optimized buffer chemistry support balanced, specific amplification. |
| Reaction Conditions / Protocol | Combine the reagent with assay-specific primers, probes and RNA template, then run a validated one-step RT-qPCR program. Optimize the freeze-drying cycle for the final formulation. |
| Components / Formulation | Glycerol-free one-step RT-qPCR enzyme system, lyophilization-compatible reaction buffer and dUTP/UDG carryover-control chemistry. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 200 reactions; 1,000 reactions; 5,000 reactions. |
| Product Form | Glycerol-free one-step RT-qPCR probe kit. |
| Quality Control | Amplification efficiency, freeze–thaw stability, accelerated storage stability and matrix compatibility are evaluated. |
| Key Features | Glycerol-free formulation; high amplification efficiency; strong storage stability; complex-sample compatibility; dUTP/UDG contamination control. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | Reported Cq variation remained within ±0.5 after temperature-stress and freeze–thaw testing. |
| Compatibility | Suitable for probe-based real-time PCR instruments and custom lyophilized RNA assays. |
| Application Notes / Precautions | Validate primers, probes, excipients and drying conditions in the complete assay configuration. |
For research use only, not for clinical use.
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