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| Product Name | Glycerol-Free Lyophilizable One-Step RT-qPCR Probe Kit |
| Catalog No. | MDKPK-0044 |
| Description | A glycerol-free one-step probe RT-qPCR kit containing lyophilization excipients for development of stable freeze-dried RNA detection reagents. |
| Intended Use | Development of lyophilized one-step RT-qPCR assays for RNA targets, including multiplex respiratory pathogen testing. |
| Principle / Technology | Optimized glycerol-free enzymes, protective excipients and dUTP/UDG carryover-control chemistry support freeze-drying while maintaining sensitive one-step probe amplification. |
| Detection Method | Fluorescent probe-based real-time RT-qPCR. |
| Sample Type | RNA templates used in singleplex or multiplex probe assays. |
| Performance Range / Specifications | 200 or 1,000 reactions; supports multiplex amplification and approximately 27-minute cycling after reconstitution. |
| Sensitivity / LOD | Designed for high-sensitivity amplification across multiple assay systems. |
| Specificity | Hot-start enzymes and optimized buffer chemistry support specific probe amplification after freeze-drying. |
| Reaction Conditions / Protocol | Combine with assay-specific primers/probes and apply a validated lyophilization cycle. Reconstitute the dried reagent, add sample and run a compatible one-step RT-qPCR program. |
| Components / Formulation | Glycerol-free one-step RT-qPCR probe reagent containing optimized enzymes, buffer, dUTP/UDG carryover-control chemistry and lyophilization excipients. |
| Storage Conditions | Store the liquid reagent at −30 to −15°C. |
| Package Specifications | 200 reactions; 1,000 reactions. |
| Product Form | Glycerol-free lyophilizable one-step RT-qPCR probe kit. |
| Quality Control | Post-lyophilization appearance, amplification performance, high-temperature stress and long-term room-temperature stability are evaluated. |
| Key Features | Glycerol-free; includes lyophilization excipients; multiplex compatible; high sensitivity and specificity; approximately 27-minute amplification; carryover contamination control. |
| Shipping Conditions | Ship the liquid reagent at or below 0°C. |
| Expiration Date / Stability | After lyophilization and vacuum packaging, reported performance remained stable for 60 days at 50°C and 360 days at 25°C. |
| Compatibility | Suitable for developing lyophilized fluorescent probe RT-qPCR assays. |
| Application Notes / Precautions | Lyophilization performance depends on the final primer/probe system, fill volume, container and drying process; validate the complete assay configuration. |
For research use only, not for clinical use.
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