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| Product Name | Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Activity Assay Kit, 50T/48S, 25T/24S |
| Catalog No. | SCAK-YJL-0026 |
| Detection Method | UV spectrophotometry, Spectrophotometer |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a crucial enzyme in the glycolytic pathway, catalyzing the oxidation and phosphorylation of glyceraldehyde-3-phosphate. Beyond its classical role in energy production, GAPDH is involved in diverse non-metabolic processes, including apoptosis, DNA repair, and gene transcription. This kit employs a coupled enzymatic reaction that links NADH production to a colorimetric signal, allowing for precise and continuous monitoring of GAPDH activity in various biological samples. |
| Product Application | • Fundamental research on glycolysis, cellular bioenergetics, and metabolic reprogramming (e.g., in cancer). • Assessment of cellular viability and metabolic activity. • Use as an internal activity reference for normalizing other enzyme assays in tissue/cell extracts. • Investigation of the non-canonical, “moonlighting” functions of GAPDH. |
| Product Advantages | • Kinetic Readiness: Designed for real-time kinetic measurement, providing a dynamic view of enzyme activity. • Excellent Linearity: Offers a wide dynamic range for accurate quantification across high and low activity samples. • Dual-Format Options: Available in two sizes (50T/48S and 25T/24S) to suit different throughput needs and prevent reagent waste. • Minimal Interference: The coupled assay system is highly specific, reducing background from sample matrices. |
For research use only, not for clinical use.
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