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| Product Name | Fluorescent LAMP/RT-LAMP Kit |
| Catalog No. | MDKPK-0047 |
| Description | A warm-start fluorescent LAMP and RT-LAMP kit for rapid one-step isothermal detection of DNA or RNA targets. |
| Intended Use | Real-time fluorescent LAMP and RT-LAMP assays. |
| Principle / Technology | A 2× master mix combines optimized buffer, dNTPs, warm-start Bst polymerase and warm-start reverse transcriptase. A separately supplied intercalating dye enables real-time fluorescence detection. |
| Detection Method | Real-time fluorescence using the SYBR/FAM channel. |
| Sample Type | Purified DNA or RNA and compatible crude lysates, including whole blood, saliva and oral swabs. |
| Performance Range / Specifications | 100 or 500 reactions; compatible with room-temperature reaction setup for up to 3 hours in the reported evaluation. |
| Specificity | Warm-start control improves reaction specificity during room-temperature setup. |
| Reaction Conditions / Protocol | Add primers and template to the 2× master mix, add the fluorescent dye at the instrument-appropriate final concentration, and incubate under validated LAMP or RT-LAMP conditions. |
| Components / Formulation | 2× fluorescent LAMP/RT-LAMP master mix containing buffer, dNTPs, Bst polymerase and reverse transcriptase; 50× LAMP fluorescent dye in DMSO. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions; 500 reactions. |
| Product Form | Two-component fluorescent LAMP/RT-LAMP kit. |
| Quality Control | Amplification performance, template compatibility, room-temperature setup stability and instrument compatibility are evaluated. |
| Key Features | One-step LAMP/RT-LAMP; warm-start enzymes; broad template compatibility; room-temperature setup; real-time fluorescent detection. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with common real-time PCR instruments; use the SYBR/FAM channel and select no passive reference. |
| Application Notes / Precautions | The recommended dye final concentration is 0.1× to 1× depending on the instrument. |
For research use only, not for clinical use.
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