- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Fish Gelatin Blocking Buffer, 1x Solution |
| Catalog No. | ATR-B-YJL-0172 |
| Description | A cold-water fish skin gelatin blocking reagent providing an alternative protein blocker for immunohistochemistry and immunofluorescence applications where mammalian protein blockers may cause cross-reactivity. |
| Intended Use | Blocking non-specific binding in IHC, ICC, and IF protocols, especially when using anti-mouse or anti-rabbit secondary antibodies on tissues with high endogenous immunoglobulin content. |
| Principle / Technology | Fish gelatin is phylogenetically distant from mammalian proteins, minimizing cross-reactivity with anti-mammalian secondary antibodies and reducing background staining. |
| Detection Method | Fluorescence microscopy, immunohistochemical staining intensity quantification |
| Sample Type | Frozen and FFPE tissue sections, cultured cells on coverslips, whole-mount specimens |
| Performance Range / Specifications | Background fluorescence reduction >85% compared to serum-based blockers |
| Sensitivity / LOD | N/A |
| Specificity | No cross-reactivity with anti-mouse, anti-rabbit, anti-goat, or anti-rat secondary antibodies |
| Reaction Conditions / Protocol | Apply to specimen for 30-60 minutes at room temperature; use at 2-5% final concentration; wash with PBS-T. |
| Components / Formulation | Cold water fish skin gelatin, Tris-buffered saline, 0.05% sodium azide |
| Storage Conditions | 2–8°C |
| Shelf Life | 12 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid |
| Quality Control | Species cross-reactivity panel verification, protein concentration, sterility |
| Key Features | No mammalian protein cross-reactivity; ideal for multi-species IHC; compatible with fluorescence and chromogenic detection |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
